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RNA integrity is a critical factor in obtaining meaningful gene expression data. Commercially available products, such as RNAlater (Ambion, Austin, TX, USA) that do not require cold chain logistics or complicated sample recovery protocols. temperature on the benchtop inside a sealed-moisture barrier bag including a
Significantly, the recommended method for utilizing RNAlater™ involves initial storage for portion of liver was placed in a labeled whirl pack bag and submerged in liquid nitrogen. either by snap-freezing or in RNAlater™ following the manufacturer's protocol. . RNA Stabilization Solution; Austin, TX: Ambion, Inc; 2007.
These protocols are for purification of total RNA (>200 nt) or total RNA including miRNA (and other Waste bag holder .. Trademarks: QIAGEN®, Allprotect®, QIAsymphony®, TissueRuptor® (QIAGEN Group); RNAlater® (Ambion, Inc);.
30 Oct 2007 sonal luggage on an aeroplane or with a courier service. Three tested by preserving tissue samples in RNAlaterTM (Ambion) or DMSO Neither RNAlater or DMSO salt-saturated solution are con- trolled by any . Littorinid DNA was not compared, because a different DNA extraction protocol was used.
1 May 2006 RNA extraction protocol from the Shock Aging Center. Pour RNAlater into a 14ml tube (enough for 500ml per 1.5ml tube and 2ml per weigh. boat). Place any tubes or tips that are used for phenol or chloroform in a plastic bag During spin, obtain ice, a 1.5ml tube of “Ambion" dH 2 O, and 1.5ml tubes.
17 Oct 2013 We developed a protocol to (i) select tissues of high cellular purity on the .. To minimize contamination, take one tube at a time from the bag with can be stabilized by immersion in RNAlater (Ambion) or Allprotect tissue
Invitrogen™ RNAlater™ Stabilization Solution is an aqueous, nontoxic tissue . standard RNA isolation protocols are also compatible with RNAlater solution.
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