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21 May 2015 Reader Comments (0) · Media Coverage (0); Figures .. cloned into the lentiGuide-Puro vector (Addgene plasmid # 52963), according to the . Relative live cell numbers were then accurately quantitated by the CellTiter-Glo
23 Mar 2017 guidelines for determining screening parameters and maintaining sufficient coverage. . Systematic and high-throughput genetic perturbation technologies within live model organisms are .. lentiGuide-Puro (Addgene, cat. no. Mouse SAM Library, Puro optimized, 3-plasmid system (Addgene, cat. no.
18 Sep 2014 LentiGuide-Puro has a ~ 100-fold increase in functional viral titer over both sub-libraries, yielding 6 sgRNAs per gene, for higher coverage.
Zhang lab lentiviral vector, also known as pLentiGuide-Puro, for expressing a single guide RNA (sgRNA) in Cas9-expressing cells. lentiGuide-Puro.dna. ? BsmBI (9882) BsmBI (7997) polIII terminator polIII terminator lentiGuide-Puro 10,183 bp.
stream signaling responses such as phosphatidylinositol (3,4,5)- lives on the order of 30–60 s. CG-3?) cell lines were also cloned into LentiGuide-Puro.
14 Sep 2017 sgRNAs were cloned into lentiGuide-Puro (Feng Zhang Lab) or with the GeCKO CRISPR lentivirus library at a MOI of 0.2 (200? coverage of the library). Matrigel-coated glass bottom dish for 24 h, followed by live imaging.
30 Nov 2015 To achieve robustness, we used a coverage so that ?100? different E9.5 .. analysis, cells were first stained with Live/Dead Blue (Life Tech, 1:100) and . (Supplementary Table 8) and cloned into lentiGuide-Puro vector.
18 Jun 2016 coverage. Systematic and high-throughput genetic perturbation technologies within live model .. lentiGuide-Puro (Addgene, cat. no. 52963).
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Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9.
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