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Cells were then stained with FITC-tyramide solution according to the manufacturer's instructions (Invitrogen). Coverslips were washed three times (5 min each) in 1 ml of PBS-T between each staining step. For F-actin staining, 200 ?l of Alexa 488 phalloidin (5 ?l of 6.6 ?m phalloidin stock solution in methanol added to 200 ?l
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Evaluating xylene-free processing for routine use. An Evaluation of Xylene-free Processing of Tissues From the Central Nervous System Using the PELORIS Dual Retort Rapid Tissue Processor
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Leica IF / IIF / IIIF and Supplementary Instructions Posted 12-30-'06. The next page contains information on this camera. If the image below looks like your camera, click below to see the full manual.
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