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Pcr2 1 vector pdf: >> http://hfu.cloudz.pw/download?file=pcr2+1+vector+pdf << (Download)
Pcr2 1 vector pdf: >> http://hfu.cloudz.pw/read?file=pcr2+1+vector+pdf << (Read Online)
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1. Cloning of DNA fragments in plasmid vectors. Please note that today's work will be under S1 condition. Make sure that you follow the. S1 rules at all times. In today's lab commercially available cloning vector called pCR2.1-TOPO. For more background information on today's protocol, please refer to the PDF file on the.
8 Apr 2004 TOPO TA Cloning® reagents (Box 1) and a box with One Shot® Chemically . Vector). • Incubate 5 Minutes at Room Temperature. • Transform TOPO® Cloning Reaction into One Shot® Competent Cells. • Select and Analyze 10 White or Light Blue Download manuals in Adobe® Acrobat® (PDF) format.
Parental bacterial expression vector for TOPO® TA cloning of PCR products.
Alt Name: pCR2.1 TOPO. Analyze: Sequence. Plasmid Type: Bacterial Expression. Promotor: Plac. Cloning Method: TOPO Cloning. Size: 3900. 5' Sequencing 1 Primer: M13 reverse. 5' Sequencing 1 Primer Sequence: T7. Bacterial Resistance: Ampicillin. Stable: Unspecified. Constitutive: Unspecified. Viral/Non-Viral
The TA Cloning Kit with pCR2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. The TA Cloning Kit uses the pCR2.1 cloning vector and New! Save technical documents to your personal library. Open a PDF, then look for the "Save to Library" icon.
LacZ? fragment: bases 1-547. M13 reverse priming site: bases 205-221. Multiple cloning site: bases 234-357. T7 promoter/priming site: bases 364-383. M13 Forward (-20) priming site: bases 391-406 f1 origin: bases 548-985. Kanamycin resistance ORF: bases 1319-2113. Ampicillin resistance ORF: bases 2131-2991.
The linearised TOPO TA vector, pCR2.1-TOPO is specially designed for cloning such. DNA fragments. It has single overhanging deoxythymidine (dT) residues at its 3' ends adjoining the sequence 5'-CCCT-3' (see Figure 2). This allows base-pairing of the A and T residues between vector and PCR products. Ligation of the
7 Apr 2004 1. Introduction. Overview. Purpose. The TA Cloning® Kit with pCR®2.1 provides a quick, one-step cloning strategy for the direct insertion of a PCR product into a plasmid vector. Advantages. Using the TA Cloning® Kit: • Eliminates any enzymatic modifications of the PCR product. • Does not require the use
LacZ? fragment: bases 1-571. M13 reverse priming Portions of this vector have not yet been completely sequenced. If you suspect an error in T7 Promoter. M13 Forward (-20) Primer. M13 Forward (-40) Primer. Not I. Xho I. Nsi I Xba I. Apa I. +1. pCR®2.1-TOPO. 3.9 kb. M13 Reverse Primer. lacZ? ATG. Kpn I. Sac I. Spe I.
TOPO® TA Cloning® reagents (Box 1) are listed in the following table. Note that the user must supply Taq polymerase. Store Box 1 at ?30°C to ?10°C. Item. Concentration. Amount. 10 Rxns. 25 Rxns. 50 Rxns. pCR™2.1-TOPO® vector or. pCR™II-TOPO® vector. 10 ng/µL plasmid DNA in: 50% glycerol. 50 mM Tris-HCl, pH
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