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8 Nov 2014 Are these data regularly collected at and/or below a national level? Are these data collected manually or electronically? RESEARCH REPORTS . antinuclear, anticardiolipin, anti-thyroid and anti-smooth muscle antibodies). Monoclonal gammopathies. Immune thrombocytopenia. Porphyria cutanea tarda.
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If muscle completes successfully, it will create a file seqs.afa containing the alignment. By default, output is created in "aligned FASTA" format (hence the .afa extension). This is just like regular FASTA except that gaps are added in order to align the sequences. This is a nice format for computers but not very readable for
MUSCLE stands for MUltiple Sequence Comparison by Log-Expectation. MUSCLE is claimed to achieve both better average accuracy and better speed than ClustalW2 or T-Coffee, depending on the chosen options.
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PCR amplification and sequencing. Whole genomic DNA was extracted from muscle tissue of. 94 species (Appendix 1, available at www.dnabarcoding.ca/ version 2.1.1 (Applied Biosystems) and manually edited. Results. Strong PCR products were generated with the 16S primers for all but two samples indicating that
MUSCLE is a program for creating multiple alignments of amino acid or nucleotide sequences. A range of options is provided that give you the choice of optimizing accuracy, speed, or some compromise between the two. Default parameters are those that gave the best average benchmark accuracy in my tests. However
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