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9 Apr 2013 The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Huggett JF(1), Foy CA, Benes V, Emslie K, dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to
20 Sep 2013 Measuring RNA by reverse transcription real-time quantitative PCR (RT-qPCR) is an established approach for investigating gene expression and viral .. dPCR versus UV quantification. .. (2013) The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.
When it comes to DNA research, scientists often find themselves weighing digital PCR (dPCR) against the more established quantitative real-time PCR On one hand, they value qPCR for its known workflows and the Minimum Information for the Publication of Quantitative Real-time PCR Experiments (MIQE1) guidelines
25 May 2017 The Minimum Information for the Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines and related articles published thereafter define a rigorous methodology for designing qPCR experiments that assures publication of reproducible and high quality data. The consequence of ignoring
Duplex assays were developed for two molecular techniques—qPCR and droplet digital PCR (ddPCR). After optimization, both qPCR and ddPCR assays have high linearity and quantitative correlations for standards. Comparisons of the two techniques showed that qPCR has higher sensitivity, a wider linear dynamic range
26 Feb 2009 15 Nonstandard abbreviations: qPCR, quantitative real-time PCR; MIQE, Minimum MIQE. (Minimum Information for Publication of Quantita- tive Real-Time PCR Experiments, pronounced mykee) is modeled on similar guidelines drawn up for DNA .. zation, the type of sample (e.g., in situ tissue vs log-.
Furthermore, the increasing popularity of digital PCR has resulted in the publication of MIQE guidelines for digital PCR (6) and the Whole vs. microdissected; Sample storage conditions and duration; Fixed - How fixed, how old?
This is where the MIQE guidelines will really help. Guidelines for minimum information required for publication of a digital PCR (dPCR) experiment were published by JF Huggett et al. in 2013. These were a follow-up to minimum information requirements for publication of real-time PCR experiments (qPCR) suggested by SA
The MIQE guidelines and the resulting scientific validity will be supported by more and more researchers, biological journals, academic and commercial institutions. Today in July 2016 we count more than 4600 citations for the MIQE guideline applied in qPCR and around 160 citations for the digital PCR (dPCR) MIQE
Similar to the MIQE guidelines that state the minimum information for publication of quantitative real-time PCR and digital PCR experiments (Bustin et al., 2009; Huggett et al., 2013), a template table (Table 4) is provided in which developers of qualitative qPCR methods can find all relevant experimental conditions and

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March 2018