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2 Mar 2015
The design of an effective, functional guide RNA is critical to achieving efficient gene knockout. All Edit-R predesigned guide RNA (synthetic crRNA and lentiviral sgRNA) are designed with a validated algorithm to improve the likelihood of functional knockout, not just double
GenScript's free, online gRNA design tool lets you instantly design high-specificity guide RNA (gRNA) sequences for CRISPR genome editing.
GenScript CRISPR Plasmid Repository. GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol or ID on our gRNA Database.
Once you have selected your CRISPR components and method of delivery, you are ready to select a target sequence and design your gRNA. grna design process diagram. Know your cell line/organism and genomic sequence. When possible, you should sequence the region you are planning
This groundbreaking technology has revolutionized genetic engineering through its ease of use, allowing targeting of any genomic loci simply by designing a 20 base pair RNA oligonucleotide. Guide RNA (gRNA) or short guide RNA (sgRNA) is an important component of the CRISPR Cas9 system and many considerations
3 May 2017 Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.
Predesigned guide RNAs for gene editing experiments are designed using a validated functional algorithm, are available for human, mouse and rat model organisms and are easy to order.
Genome-wide gRNA database for CRISPR/Cas9 genome editing. Search for validated gRNA sequences targeting any mouse or human gene.
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