
Overlap extension pcr protocol pdf viewer
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The product of the overlap extension PCR is used to transform competent Escherichia coli cells. Phusion
Megaprimer whole plasmid cloning aka MEGAWHOP cloning aka Overlap Extension PCR cloning Use modified 25ul Phusion protocol . 5 ul 5x Buffer
The Big Picture . Two stretches of linear DNA with homologous ends can be combined using this technique. Useful papers: Gene splicing and mutagenesis by PCR-driven
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