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Nucleofector™ 2b Manual. 2.1 Restrictions. Medical use restrictions. The Nucleofector™ Technology is intended for research and investigational use by professionals only. Please note that Lonza's Nucleofector™ Techno- logy is not intended to be used for diagnostic purposes, for testing or treatment in humans. License
8 Feb 2010 Chapter 2. 2. Operating instructions. 4-15. 2.1. Restrictions. 4. 2.2. Maintenance. 4. 2.3. Safety instructions – please read carefully! 5. 2.4. Waste disposal. 7. 2.5. Nucleofector® II components. 7. 2.6. Set-up instructions. 8. 2.7. Instructions for using the Nucleofector® II Device. 9. 2.7.1. Main menu and program
Application. This protocol enables you to optimize the Nucleofection® Conditions for the transformation of Program 1. Program 2. Program 3. Program 3. Program 4. Program 5. Program 6. Program 7. Step 1: For each bacteria strain try 7 different Nucleofector®. Programs*. program see Nucleofector® II Manual, 2.7.3).
Chapter 2. 2. Operating instructions. 4-13. 2.1. Restrictions. 4. 2.2. Maintenance. 4. 2.3. Safety instructions – please read carefully! 5. 2.4. Nucleofector™ components. 6. 2.5. Set-up instructions. 7. 2.6. Instructions for using the Nucleofector™ II Device. 7. 2.6.1. Main menu and program execution. 7. 2.6.2. Selection of
1 One 4D-Nucleofector® Core Unit including two side panels. 2 At least one Functional Unit, including two side panels. X Unit: Comprising two retainers for 100 µl Nucleocuvettes and one retainer for 16-well Nucleocuvette® Strips. 3 Power cable. 4 HV/data cable. — 4D-Nucleofector® Manual. Figure 2.1: 4D-Nucleofector®
8 Feb 2010 2.5 Nucleofector™ II Device. Components. The Nucleofector™ II Device is delivered with the following components: — 1 Nucleofector™ II Device. — 1 Power cord. — 1 Nucleofector™ II Manual. Front panel of the Nucleofector™ II Device. 1 Power button. 2 Graphic display. 3 “Up" button. 4 “Down" button.
2B). pMaster vector-derived iPS cell lines demonstrated normal karyotypes, with the 3 µg of. pMaster DNA was transfected by using the Lonza Amaxa Nucleofector, program A-024. Manipulating the Mouse Embryo: A Laboratory Manual. 15 functional groups (Figure 2B & C). Most of the chan- Nucleofector® Kit V with the
Amaxa® Nucleofector® Technology is a highly efficient non-viral method for transfection. It is based on 2 components: the Nucleofector® Device that delivers unique electrical parameters, and Nucleofector® Kits. These contain Lonza certified cuvettes, pipettes, Nucleofector® Solution and Supplement. Each solution and
2. Operating instructions. 2.4. Nucleofector™ components. The Nucleofector™ is delivered with the following components. › 1 Nucleofector™. › 1 Power cord. › 1 Warranty and Registration Card. (please complete and return). › 1 Nucleofector™ manual. Fig. 1. Front side of the Nucleofector™. 1 Power indicator. 2 Display (4
2. BioResearch. Amaxa™ 4D-Nucleofector™ Optimization Protocol for Cell Lines. Optimization Guidelines. The initial optimization experiment is comprised of 48 . 2.15 Start Nucleofection™ Process by pressing the “Start" on the display of the 4D-Nucleofector™ Core Unit (for details, please refer to the device manual).
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