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oped (Sepax, Biosafe S.A., Eysins/Nyon, Switzerland). This system consists of a computer-controlled with a manual system based on blood bags and using hy- droxyethyl starch (HES) (n. 19 for each technique). plasma with white blood cell (WBC) fraction was trans- ferred to a processing bag (Stem Cell Processing Sys
Sepax. ®. 2 cell processing device. Sepax 2 is the new generation of Sepax technology for automated adult stem cell processing. This mobile, closed capability system efficiently processes umbilical (centrifugation) and component transfer through displacement of the syringe piston. Biosafe Medical Device Int. Trading.
Cryoprotectant solution is then added directly to the freeze bag. In the manual method, buffy coat and white cell rich plasma layer is collected after first centrifugation step and the white cells are separated from the plasma after the second centrifugation step. Cryoprotectant solution is then added to the cells before transfer to
Mar 30, 2012 The minimum final volume used was 40 mL. The re-suspension medium was as described for the manual isolation process. FIG. 1. Schematic description of the isolation process. The adipose tissue sample is digested with collagenase in a transfer bag. The bag is connected to the Sepax kit and the kit is
The fundamental scientific technology relies on a separation chamber that provides both separation through rotation of the syringe chamber (centrifugation) and component transfer through displacement of the syringe piston. An optical sensor measures the light absorbency of the separated components and manages the
Nov 16, 2016 Manual processing is when cord blood is handled in the laboratory by trained technicians. These technicians should flow cabinet. Automated processing is when the cord blood collection bag goes into a functionally closed device like the Sepax 2 made by Biosafe or the AXP made by Cesca Therapeutics.
Sepax 2. A versatile technology platform, the new Sepax 2 is a fully-automated, mobile, GMP compliant system for the efficient and reproducible processing of umbilical cord blood, bone marrow, peripheral blood or other blood-like material. In keeping with industry best practice and trends, the Sepax 2 is a tool to
Sepax® 2. SEPAX S100 (Sepax® 2). It is a cell separator, fully automatic, closed-loop and sterile, it allows a complete and efficient separation of cord blood, of blood components (red blood cells, buffy coat, plasma) through the rotation of the centrifuge both the transfer of the same through the movement of the piston.
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This procedure must ensure high cell recovery, cell viability and RBC depletion by reducing the UCB units to a standard volume. Although the first described procedure for this purpose was manual and used the sedimentation agent hydroxyethyl starch (HES), the current trend is to use automated systems. Sepax (Biosafe
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