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Pharmacology and Toxicology. - Highly sensitive indicator of a drug's activity (pharmacology) and toxicity (toxicology) in cell culture or test animals. - Screen or optimize drug candidates prior to costly clinical trials. Diagnostics. - Potential to diagnose clinical conditions by detecting gene expression patterns associated with
Microarrays. A microarray is a pattern of ssDNA probes which are immobilized on a surface (called a chip or a slide). The probe sequences are designed and placed on an array Like Southern & northern blots, microarrays use hybridization to detect a specific DNA or . s/activity3/activity3_manufacturing_background.pdf.
Technique. Gene microarray technology rests on the ability to deposit many (tens of thousands) different DNA sequences on a small surface, usually a glass slide (often referred to as a “chip"). The different DNA fragments are arranged in rows and columns such that the identity of each fragment is
18 Mar 2004 Microarrays are currently used to do many different things: to detect and measure gene expression at the mRNA or protein level; to find mutations and to genotype; to (re)sequence DNA; to locate chromosomal changes (CGH = comparative genomic hybridization), and more. There are many different ways.
DNA Microarray Technology. What is it and how is it useful? Mandana Sassanfar and Graham Walker. Department of Biology. Massachusetts Institute of Technology. Cambridge, MA. NOTE: The following material was developed for high school audiences familiar with the structure of DNA and the central dogma of molecular
The DNA chip technology (or DNA microarray technol- ogy) is a reverse dot blot technique where the number of spotted oligonucleotides with known sequence can vary between several thousand to even a hundred thousand. Thus this technology is a hybridization based nucleic acid detec- tion system, where thousands of
Hybridization-based methods for measuring nucleic acid sequences have been used as a standard technique for decades [7]. The most widely accepted and understood standard for measuring gene expression is a hybridization-based assay called the. Northern blot [8]. Microarrays are derived from blotting assays. Thus
microarray technique. 1.1 Principles of microarray analysis. Despite the variety of technical solutions that have been developed for performing microarray analysis, all are miniaturized hybridization assays for studying thousands of nucleic acid fragments simultaneously. All microarray systems (Fig 2) share the following key
18 Oct 2013 DNA microarray technology (also known as DNA arrays, DNA chips or biochips) represents one of the latest breakthroughs and indeed major achievements in experimental molecular biology. This novel technology, which started to appear during the second half of the 1990s, has historically evolved from.
Max F. Perutz Laboratories (MFPL). University of Vienna and Medical University Vienna. Austria. The originality of this thesis has been checked in accordance with the University of Turku quality assurance system using the Turnitin OriginalityCheck service. ISBN 978-951-29-6354-6 (PRINT). ISBN 978-951-29-6355-3 (PDF).
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