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Gecko2 lentiguide puro: >> http://gtk.cloudz.pw/download?file=gecko2+lentiguide+puro << (Download)
Gecko2 lentiguide puro: >> http://gtk.cloudz.pw/read?file=gecko2+lentiguide+puro << (Read Online)
LentiCRISPRv2 and lentiGuide-Puro: Lentiviral CIRSPR/Cas9 and single guide RNA GeCKO Library Product Information Catalog Number ASK-7800 CRISPR
GeCKO v2 Human CRISPR Library B (lentiGuide-Puro) GeCKO v2 Mouse CRISPR Library B (lentiGuide-Puro) Addgene# 1000000053, Library B Addgene# 1000000052, Library B
To further increase viral titer, we also cloned a two-vector system, in which Cas9 (lentiCas9-Blast) and sgRNA (lentiGuide-Puro) are delivered using separate viral
(lentiGuide-Puro) are delivered using sequences) and libraries further expand the GeCKO toolbox for sample handling using a tablet To the Editor:
with the GeCKO library and grown in the presence of lentiGuide-Puro) RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes
Supplementary Figure 2 | Sequencing of human GeCKOv2 libraries cloned into lentiCRISPRv2 and lentiGuide-Puro. Histograms of sgRNA representation after cloning each
in Primary Immune Cells to Dissect annealed and ligated to lentiGuide-Puro using standard protocols. sgRNA target sequences were taken from the GeCKO
CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for methods 39 and was modified from the GeCKO individual-gRNA (lentiGuide-Puro-2
lentiGuide-Puro New GeCKO libraries target an increased number of genes with uniform coverage (6 sgRNAs per gene divided into sub-libraries with 3 sgRNAs per gene).
Improved vectors and genome-wide libraries for CRISPR packaging and choice of guide sequences in our original GeCKO lentiGuide-Puro)
recently published methods39 and was modi?ed from the GeCKO individual-gRNA cloning scheme.35,40 First, the con was cloned into lentiGuide-Puro with In-Fusion
recently published methods39 and was modi?ed from the GeCKO individual-gRNA cloning scheme.35,40 First, the con was cloned into lentiGuide-Puro with In-Fusion
vector lentiviral GeCKO system cPPT npg EF1a lentiGuide-Puro Eric A Stone1-3 1. 2 2013). 8.2 kb 10 1 Improved vectors and genome-wide
I've used BsmBI from NEB to digest LentiGuide-puro vector (for CRISPR) multiple times: 1ul BsmBI + 5ul buffer 3.1 + 2~3ug vector + water = 50ul system, 55C. I found
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