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The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium. Below are some guidelines for
Although developed in 1884, the mechanism of the Gram stain was proven only recently. It is described as differential solubility. The primary dye (crystal violet) and the mordant (Grams' iodine) react similarly in both cell types. The decolorizer, ethyl alcohol, is the most critical step. Ethyl alcohol is a nonpolar solvent, and thus
Feb 29, 2012 MW 3:45-5:00. Gram Stain Report. Introduction. The purpose of this experiment is to determine the shape and Gram stain of the bacteria under a microscope. The reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be seen through the microscope. The Gram stain is a
The gram stain, originally developed in 1884 by Christian Gram, is probably the most important procedure in all of microbiology. It has to be one of the most repeated procedures done in any lab. Gram was actually using dyes on human cells, and found that bacteria preferentially bind some dyes. The Gram stain is a
There are several objective why we should do this experiments such as knowledge of the differences between gram positive and gram negative bacteria, develop the lab skill, and be more familiar with gram staining procedure. There are also several material that we use in this experiment such as Hucker's crystal violet,
Lab Experiment 2. The Gram Stain. A one lab session experiment. In most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear. In this procedure living, potentially pathogenic bacteria are smeared on the glass slide and allowed to dry. Then the slide is heated so that the bacteria
in reactions to Gram stain are indicative of how the body responds to pathogenic bacteria. While this is an extremely useful stain for 1 each per lab table: Gram's crystal violet, Gram's iodine, and Gram's safranin stains, ethanol/acetone rinse . Unlimited Special Reports/Antibiotics in food www.guardian.co.uk/antibiotics/.
MEDICAL MICROBIOLOGY. EXPERIMENT 1. Wang Yuyan. Bacterial stain. Living bacteria stain. Killed bacteria stain. Positive staining. Negative staining. Common stain. Single-dye stain. Special stain. Complex stain Endospore stain Capsule stain. Gram stain. Cell wall stain. Acid fast stain. Methods for staining bacteria
Lab Report #1. Exercise 7B Gram Staining an Unknown. The report is worth 100 points. It should be 2-3 pages long, typed, 12-point font and double-spaced. It should contain an introduction, procedure (materials and methods), results, discussion and literature cited. Label each section in your report. You must cite.
A. Gram Stain. The previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria. Although simple stains are useful, they do not reveal details about the bacteria other than morphology and arrangement. The Gram stain is a differential stain commonly
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