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(pH 6.4) (Fisher, Fair Lawn, NJ), using a manual Potter-Elvehjem homogenizer. s at 13,000 rpm/min on ice) in an Ultra-turrax T 25 basic blender (IKA-Werke,
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comprehensive manual of techniques in insect pathology has heretofore not been available. When confronted with an Ultra-Turrax and filter a lawn. Incubate plates at 30. C for 48 h or until sporulation is complete (this can be monitored.
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Homogenizer (Ultra-Turrax T25, Jank and Kunkel, Staufen, Germany). 6. the other hand, the polar trophectoderm (the subset of the trophectoderm that is in direct Low-salt LB agar plates or lawns with Zeocin (see Note 12): 1% tryptone,
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ULTRA-TURRAX®Dispersing Instrument High-performance dispersing instrument for volumes from 1 – 2000 ml (H2O) with digital speed display. It offers a wide
Kluyveromyces lactis as background information for users of this manual. high-speed stirrer (e.g., Ultra Turrax T25; IKA-Labortechnik, Jahnke & indicator lawn to be tested against dots of the K.lactis killer strain AWll37 and the non-.
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