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In pharmacognosy, a typical protocol to isolate a pure chemical agent from natural origin is bioassay-guided fractionation, meaning step-by-step separation of extracted components based on differences in their physicochemical properties, and assessing the biological activity, followed by next round of separation and
9 Jan 2014 (2014) Bioassay-Guided Fractionation of Extracts from Codiaeum variegatum against Entamoeba histolytica Discovers Compounds That Modify Expression of Ceramide Biosynthesis Related Genes. PLoS Negl Trop Dis 8(1): e2607. https://doi.org/10.1371/journal.pntd.0002607. Editor: Timothy G. Geary,
3 Jun 2017 Highlight. Endogenous unsaturated fatty acids identified from salt-treated Arabidopsis by a bioassay-guided fractionation method activate plasma membrane H+-AT.
Molecules. 2015 Aug 14;20(8):14810-32. doi: 10.3390/molecules200814810. Bioassay-Guided Fractionation of a Leaf Extract from Combretum mucronatum with Anthelmintic Activity: Oligomeric Procyanidins as the Active Principle. Spiegler V(1), Sendker J(2), Petereit F(3), Liebau E(4), Hensel A(5). Author information:
22 Feb 2016 Table 1. Antibacterial activity against V. anguillarum of solid fermentation products, EtOAc extracts, n-BuOH extracts and corresponding residues determined based on the Oxford cup method. CSVDisplay Table. Three pure compounds were isolated from the active EtOAc extracts, using the bioassay-guided fractionation method
Pharm Biol. 2017 Dec;55(1):206-211. Bioassay-guided fractionation and identification of ?-amylase inhibitors from Syzygium cumini leaves. Poongunran J(1), Perera HK(2), Jayasinghe L(3), Fernando IT(2), Sivakanesan R(2), Araya H(4), Fujimoto Y(3)(4). Author information: (1)a Postgraduate Institute of Science, University
4 Jul 2012 Frequently used terms are bioassay- or (bio)activity-guided fractionation, biochemical detection, and bioactivity screening. Most of them are mainly connected with drug discovery. Others, such as effect-directed analysis or bioresponse-linked instrumental analysis are more rooted in the environmental
For crude ethanol extracts and organic fractions tested, the bark dichloromethane phase showed a significant antibacterial effect, with MIC values ranging from 12.5 to 100 ?g/ml for most of the microorganisms tested. Thus, a bioassay-guided fractionation of this fraction was performed. This fractionation led to isolation of the
Background and Objectives: Due to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cineraria aerial parts were investigated using a bioassay guided fractionation scheme.
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