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30 Mar 2015 However, CCM3 expression in the absence of CCM2 is sufficient to . biolayer interferometry (Pall Life Sciences) revealed a similar affinity of 9.5 constructs were generated by following the protocol described in Fu et al.
18 Dec 2014 The protein products of KRIT1 and CCM2 (Krev interaction trapped 1 (KRIT1) is then followed by a “second hit" mutation acquired later in life (3,–5). . and lysed following a protocol similar to that described above for CCM2.
3 Aug 2015 The protein kinase MEKK3 interacts with CCM2, which is associated with the The haematoxylin and eosin staining procedure followed standard protocol. . Human GST–MEKK3 was purchased from Life Technologies.
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22 Aug 2011 Despite a pan-endothelial Ccm2 deletion, CCM lesions are .. Within the first week of life a vascular plexus develops at the inner . Tamoxifen-induced recombination efficiency was confirmed for the different protocols used
4 Nov 2015 Both CCM2L and CCM2 interfere with MEKK3 activation and its .. with the protocol approved by the Harvard Subcommittee for Animal Research. (Gateway System; Life Technologies) and transfected into 293 cells to
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10 Jan 2007 The present study was conducted to investigate whether CCM2 is .. directed at optimizing embryo cryopreservation protocols as the cyroprotectant used . Primers (Invitrogen Life Technologies, Burlington, ON, Canada) and
Goat polyclonal CCM2 antibody validated for WB and tested in Human. have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. Protocols. Western blot protocols

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August 2017