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17 Jul 2013 We extracted total RNA using the RNeasy Lipid Tissue Mini kit. (QIAGEN), following the manufacturer's instructions and using the . We used the online calculator at faculty.vassar.edu/lowry/rdiff.html to assess the complex with Ran-binding protein in microtubule organising centre (RanBMP), and.
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to
25 Nov 2013 source of MSC, adipose tissue is emerging as an alternative. . Committee of Leiden University Medical Centre as described before. [47]. Erasmus MC (protocol no. . using TRIzol Reagent (Life technologies) and RNeasy minikit . an adoptive transfer of human PBMCs did not demonstrate leukocyte.
Published online September 22, 2017 . suggested protocol using RNeasy Lipid Tissue Mini Kit (Qiagen GmbH, Hilden, Germany) (Mongroo et al. 2004).
PDF Version (516 KB) These pollutants are stored in adipose tissue (AT). of a material transfer agreement [Centre National de la Recherche Scientifique . Courtaboeuf, France) and from mouse AT with RNeasy Lipid Tissue Mini Kit (Qiagen). .. Mediators Inflamm 2010:513948; doi: 10.1155/2010/513948 [Online 23
Published online 2012 Aug 17. doi: 10.2337/db11-1274 In lean mice, only CD11c+ DCs were detected in adipose tissue. Adoptive transfer of naive CD4+ T cells in Rag1?/? mice led to a predominant Th1 response in adipose tissue. This protocol was approved by the Institutional Research Board of INSERM and the
Published online 2011 April 20. doi: 10.1038/nature09938 . When we used the above protocol to examine the composition of the SH-SY5Y or HEK293 .. 20-30 ?l droplets containing cells were added to the centre of poly-D-lysine (Sigma) . RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen), the ribosomal
1 Dec 2006 Besides its role in energy storage, adipose tissue, an endocrine organ, . adipocytes using QIAGEN RNeasy Lipid Tissue Mini Kit according to Acid Database Searches from the National Centre for Biotechnology V for 1 h in a transfer buffer containing 20 mm Tris, 150 mm glycine, and 20% methanol.
Published online 2016 June 6. doi: 10.1155/2016/2042107 Adipose tissue (AT) histopathology and inflammatory gene expression as well as . Mouse Cytokine Assay for plasma, according to the manufacturer's instructions. tissue from the gonadal AT depot using RNeasy Lipid Tissue Mini Kit (Qiagen, Westburg,
1 Sep 2009 information from the fat body to the peripheral tissues are currently unknown RNeasy Lipid Tissue Mini Kit according to the manufacturer's protocol. RNA samples (5 A dissecting needle was used to transfer the tissues in a Supplemental Data include one figure and can be found online at www.
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