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This Test Guideline describes an in vitro screen for chemical effects on steroidogenesis, specifically the production of 17?-estradiol (E2) and testos.
effects due to the ability of a chemical to alter steroid biosynthesis. • Cyprotex follows the EPA OPPTS 890.1550 guideline1 and the OECD test guideline. 4562 for the steroidogenesis (human cell line H295R) assay and the service can be performed under GLP or non-GLP conditions. Protocol. 'The H295R Steroidogenesis.
Paris 16 December 2009 patric.amcoff@oecd.org. OECD GUIDELINE FOR THE TESTING OF CHEMICALS. DRAFT PROPOSAL FOR A NEW GUIDELINE 4XX. The H295R Steroidogenesis Assay. INTRODUCTION. 1. The OECD initiated a high-priority activity in 1998 to revise existing, and to develop new,. Test Guidelines
28 Jul 2011 This Test Guideline describes an in vitro screen for chemical effects on steroidogenesis, specifically the production of 17?-estradiol (E2) and testostero.
28 Jan 2016 H295R OECD Test Guideline. OECD Guideline Assay. • No pre-treatment or cell stimulation. • 48 hr chemical treatment in concentration-response. • Quantification of estradiol and testosterone
28 Jul 2011 This Test Guideline describes an in vitro screen for chemical effects on steroidogenesis, specifically the production of 17?-estradiol (E2) and testosterone (T). The human H295R adreno-carcinoma cell line, used for the assay, expresses genes that encode for all the key enzymes for steroidogenesis. After an
conducted using the OCSPP Guideline 890.1550 Steroidogenesis (Human Cell Line – H295R). Assay that are submitted to Aspects of the EDSP Tier 1 Assays (OCSPP Test Guideline Series 890) in the docket; the link to this document may be found by . analysis in the OECD validation studies. If the blank has greater
28 Jul 2011 permission from the OECD. OECD GUIDELINE FOR THE TESTING OF CHEMICALS. H295R Steroidogenesis Assay. INTRODUCTION. 1. The OECD initiated a high-priority activity in 1998 to revise existing, and to develop new, Test. Guidelines for the screening and testing of potential endocrine disrupting
16 Sep 2015 Comparison to OECD Guideline. OECD Test Guideline 456 H295R. Assay. • No pre-treatment. • 48 hr chemical treatment in concentration-response. • Quantification of estradiol and testosterone. ToxCast H295R Assay. • 48 hr Forskolin stimulus. • 48 hr chemical treatment for MTC and conc-response.

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March 2018