gr.171264.113. Epub 2014 Apr 2. Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Ramakrishna S(1), Kwaku Dad AB(1), Beloor J(2), Gopalappa R(1), Lee SK(2), Kim H(1). Author information: (1)Graduate School of
7 Nov 2016 Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing. By: Bharathi We developed a method for treating human cell lines with CPP-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs that leads to endogenous gene disruption. Here we
13 Jan 2018 On Nov 1, 2017, Bharathi Suresh (and others) published the chapter: Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for for treating human cell lines with CPP-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs that leads to endogenous gene disruption.
Furthermore, this delivery method requires transfection tools. Here we show that simple treatment with cell-penetrating peptide (CPP)-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs leads to endogenous gene disruptions in human cell lines. The Cas9 protein was conjugated to CPP via a thioether
Highly efficient RNA-guided genome editing in human cells via delivery of purified CAs9 ribonucleoproteins. Genome Res. Aril 8, on line publication. (2014). Suresh Ramakrishna, et al. Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res. Aril 2, on line publication.
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H Genome Res. 2014 Apr 2. PubMed Journal. Plasmids from Article. Showing 1 to 1 of 1 entries. Show. 10, 25, 50, 100, All. entries. Search table: Previous; 1
Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing. Suresh B(1)(2), and guide RNA delivery. We developed a method for treating human cell lines with CPP-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs that leads to endogenous gene disruption.
Research article has been featured on the Cover of Genome Research Journal. in 24th issue, 2014. Title: Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. SURI LAB. Enter text · HOME · PROFESSOR · CURRENT MEMBERS · PUBLICATIONS · RESEARCH · GALLERY
Plasmid- and virus-free direct delivery of Cas9 protein and guide RNA has several advantages over the conventional plasmid-mediated approach. a method for treating human cell lines with CPP-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs that leads to endogenous gene disruption. Here we

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February 2018