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11 Jan 2012 Haploids and Doubled. Haploids in Plant Breeding. Jana Murovec and Borut Bohanec. University of Ljubljana, Biotechnical Faculty. Slovenia. 1. Introduction. Haploids are plants (sporophytes) that contain a gametic chromosome number (n). They can originate spontaneously in nature or as a result of
18 Jan 2017 Full-text (PDF) | Although several methods have been developed for producing haploid plants, the in vitro techniques are much more efficient than inter-specific hybridization or treatment with plant-growth regulators, temperature or irradiation. Androgenesis is the most universal of these techniqu
culture was investigated. Following anther culture of spring oilseed rape (Thomas et al., 1976; Keller and. Armstrong, 1977; Renard and Dosba, 1980) haploid plants were produced successfully. However, only a certain proportion of the embryoids which emerged from the anthers developed directly into plants, while a
Anther culture for haploid and doubled haploid production. Maria Antonietta Germana`. Received: 30 May 2010 / Accepted: 9 September 2010 / Published online: 12 November 2010. O Springer Science+Business Media B.V. 2010. Abstract Haploids are plants with a gametophytic chro- mosome number and doubled
Haploids are organisms with the gametic chromosome number, that is, half that of somatic cells. In seed plants, haploids can be derived from tissue culture of gametic cells (microspores that develop into male gametes borne in pollen grains or megaspores that develop into female gametes within the pistil of a flower,)
gynogenesis via ovary-ovule culture can prove to be a complementary technique in species where anther culture is inaccessible or less productive [9]. The production of haploids via in vitro gynogenesis is more tedious, less efficient in comparison to androgenesis [10]. From this it is clear that not only the microspore but,
Introduction to haploid culture • History • Types of haploid culture: in vivo and in vitro • Androgenesis: A. Anther culture- protocol, advantages, disadvantages B. Pollen culture- protocol, advantages Culture media for . Introduction to plant tissue culture: second edition- M.K. Razdan (Oxford & IBH Publishing Co. Pvt. Ltd.) 2
28 Nov 2016 techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands. Keywords: anther culture; doubled haploid (DH); forage crops; microspore culture; perennial ryegrass (Lolium perenne L.); plant breeding. 1. Introduction.
22 Nov 2012 CONTENT: CONTENT Introduction Production of Haploid plants and their uses Anther/ovule culture Protocol Chromosome elimination and it's mechanism Diploidization of haploid plants Haploid plants and their uses Factor influencing Haploid plants
In vitro androgenesis involves culture of anthers or isolated microspores. • Gynogenesis may be induced in unfertilized ovule or ovary culture. • Success of haploid cultures depends on genotype, media, and cultural conditions. • Stage of microspore development at time of culture is critical to the success of andro- genesis.
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