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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide vii. Preface. How to Use This Guide. Purpose of This. Guide. This manual is written for principal investigators and laboratory staff who conduct absolute quantification assays using the Applied Biosystems
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the applied Biosystems advantage. Built on over 10 years of real-time Compared with manual. PCR quantitation techniques Applied Biosystems. 7300 Real-Time PCR System. A Real Affordable Approach to Real-Time PCR. • Four-color detection provides the flexibility to perform a variety of applications, including gene.
This manual includes instructions and thermal cycling conditions specific for use of the Plexor™ qPCR System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR System with the Applied Biosystems 7300 and 7500 Real Time PCR Systems. Instructions are included for instrument setup, data transfer
with the Applied Biosystems 7300 and 7500 Real Time PCR Systems. Instructions are included for instrument setup, data transfer from the instrument to the Plexor™. Analysis Software and data analysis. II. Plate Preparation and Amplification. Detailed instructions describing assay setup are provided in the Plexor™ qPCR.
Two ?-actin genes of the fish Leporinus macrocephalus, referring to white and red muscle tissues, were isolated. Actin isoforms, that mainly . primer, according to the manufacturer's instructions. cDNA (2µL) was amplified using out in duplicate using an Applied Biosystem 7300. HT Real-Time PCR system (Applied
The Applied Biosystems 7300, 7500 and 7500 Fast Real-Time PCR Systems are real-time thermal cyclers covered by US patents and corresponding claims Exposure may cause discomfort if swallowed or inhaled. Read the MSDS, and follow the handling instructions. Wear appropriate protective eyewear, clothing, and
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