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5 Dec 2013 Guiding the osteogenic fate of mouse and human mesenchymal stem cells through . Control medium containing a cocktail of traditional osteogenic factors (AA2P: 50 Tohoku University Graduate School of Dentistry, and S. Takeda, Journals A-Z · Subject Pages · Protocol Exchange · Blogs · Podcasts
14 Jun 2013 USA; bDepartment of Physiology, University of Michigan School of Medicine, Ann Arbor, Michigan, USA; .. The same protocol was used for ST2 and. MC3T3-E1 cells except the osteogenic medium did not contain.
osteogenic medium (DMEM, FBS 10%, 50 ?g/ml ascorbic acid, 10 mM ?-glicerophosphate, 10 nM dexamethasone) which was changed every 3 days. After 14
7 Jul 2015
21 Feb 2013 Two commonly used osteogenic markers, alkaline phosphatase (ALP) activity and Affiliation Department of Biotechnology, Graduate School of . or in non-induction medium according to the protocol illustrated in Fig.
20 Dec 2012 Differentiation into these different lineages can be easily assessed by growing cells in specific medium and assaying for differentiation markers.
for staining protocol). Osteogenic Differentiation and Analysis of MSC Use MSC Growth Medium for the remaining well as a negative control. 4. Differentiation
4 Jun 2013 aDivision of Oral Biology and Medicine, School of Dentistry, University of . grown in osteogenic differentiation-inducing media (Odi) and .. RT-PCR, total RNA was extracted according to the manufacturer's protocol.
7 Sep 2015 The protocol was approved by the Ethics committee of the Helsinki University Osteogenic differentiation medium consisted of either FCS or PLP expansion .. the National Graduate School of Musculoskeletal Diseases and
24 Jun 2005 Increasing reports on in vitro differentiation protocols for adult .. At day 9 in osteogenic differentiation medium, it was found that <1% of the anchored . and Dr. Linda Dubin, UCLA School of Dentistry, for editorial assistance.
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