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Keyence bz-9000 manual pdf
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Perform fluorescence, bright field and phase-contrast imaging without the need for a darkroom.. As a leading supplier of factory automation sensors, measuring instruments and digital microscopes, KEYENCE develops and manufactures innovative and reliable.... There are no threads in. BZ-9000E, Kompaktes Fluoreszenz-Mikroskop HS in Modellreihe BZ-9000 von KEYENCE Deutschland. BZ-9000 Series. NEW. All-in-one. Fluorescence. Microscope. Eliminates Need For. Darkroom. Before Movement. Preview Screen (Target at 20 x magnification). A. A. B. B. After Movement. Preview Screen (Target at 20 x magnification). Simple composition just by turning mouse wheel. Raw image. Haze reduction image. This Keyence BZ-9000 BioRevo Fluorescence Microscope is in good working order with all aspects tested and fully functional. Microscope and optics have been professionally. Manual: https://drive.google.com/file/d/0Bz5srQzgsa6uNlkyN0xJbF82WlU/view?usp=sharing. Please note there is no CC or PayPal due to the. What is Local Sevrice?Keyence bz-9000 manual pdf Free car racing games download Jlg scisser lift manual Manga matrix pdf Free metal music downloads Windows 8 codec pack download. An automated analysis system for the identification of phytoplankton verse Keyence BZ 9000 fluorescence system for the. The BZ-9000 BioRevo from Keyence Corp. of America offers a new approach to conventional fluorescence microscopy. The fully integrated system perfo. A Tokai Hit temperature and CO2 gas controller allows live-cell imaging. The system greatly reduces time spent on learning operations and adjustments with the microscope. Its software package includes image stitching, z-stack, multi-color/multi-points time-lapse, movie recording, time-series brightness measurement. BZ-9000 – All-in-one Fluorescence Microscope.. The BZ-9000 (BIOREVO) represents a revolutionary approach to conventional fluorescence microscopy.. For further product information, contact Andrew Kominek, Sr. Marketing Specialist, Keyence Corporation of America, 1100 N Arlington Heights Rd, Suite 350, Itasca,. Built-in darkroom. Standard equipped with a fully-motorized operation system. Large motorized stage supports observation of entire microtiter plates. Supports fluorescence, bright field, and phase-contrast images. High-sensitivity cooled monochrome/color CCD camera. High sensitivity cooled monochrome camera. I'm shopping for microscopes that will be my "bread and butter" microscope for fluorescent and bright field imaging in my lab. I was planning on getting a standard inverted fluorescent scope from Zeiss, but I recently had the opportunity to demo the Keyence BZ-X700, and was surprised by how much I liked it. Anyway, now. series instruction manual read this manual before using the product in order to achieve maximum performance nearest keyence dealer1 digital photoelectric sensor psn10 series instruction manual read this instruction manual before using the product in order to achieve maximum performance keyence bz 9000 manual pdf. ①システムの起動と終了. Page 3. Page 4. ②観察・撮影をする. Page 5. Page 6. ２-①観察倍率を変更する. Page 7. ２-②位置決めを行なう（X-Y軸移動）. Page 8. Page 9. Page 10. ２-③フォーカスを調整しする。 Page 11. Page 12. ２-④ナビゲーション機能を使用する. Page 13. Page 14. ２-⑤画像連結撮影を行なう. Page 15. Page 16. Modellreihe BZ-9000. NEU. Unverbindliche Aufnahme Ihrer Proben! Revolutionäre Fluoreszenz-Mikroskopie. Inklusive Hellfeld & Phasenkontrast. • Tiefenscharfe Bildzusammensetzung (S.12). Bei der Konzeption des Fluoreszenz-Mikroskops “Biozero" hat KEYENCE die Grundlagen der Fluoreszenz-Mikroskopie und die. PZ- Keyence Bz 9000 Manual Pdf - edardispa.files.wordpress.com Keyence Bz 9000 Manual Pdf Sample images were acquired using a Biorevo BZ-9000 acquired using a Pulse-SIM BZ-X700. as advised in the instruction manual. Keyence Fiber Optic Sensor Manual instructions manual. KEYENCE CZ-V20 series RGB. BZ-9000 - All-In-One Fluorescence Microscope by Keyence Corporation. The BZ Series is a fully-integrated fluorescence microscope which incorporates all of the components of a conventional fluorescence microscopy system, but it takes up 50% less. Teledyne Isco - Model 2101 - Field Wizard Module User Manual. Additionally, one of the specimens was documented with a Keyence BZ-9000 fluorescence microscope. The specimen was subsequently dissected to document all appendages in detail. UV light (377 nm) was used for illumination, consistent with the specimen's autofluorescence capacities. For high-resolution images,. Fungal genomic DNA was extracted using Plant Genomic DNA Extraction Miniprep System (Viogene) following the manufacturer's instruction... Mycelia were viewed under a BZ-9000 epifluorescent microscope (KEYENCE) or a FV1000-KDM confocal laser scanning microscope (Olympus) using a 100. This study was approved by the Ethics Committee of Osaka University Graduate School of. Medicine in accordance with the Declaration of Helsinki. The ethics committee did not require written informed consent from each patient for this study. To investigate the time-dependent change in NETs appearance in bronchial. fluorescence microscope (Keyence BZ9000), and examined using ImageJ software after manual delineation of the soma margins. Excitatory synaptic density was analyzed as previously described (Tsujimura et al., 2015). Briefly, hippocampal neurons were subjected to immunocytochemistry using anti-DLG4, anti-SLC17A7,. HSオールインワン蛍光顕微鏡BZ-9000の商品情報はこちら。全商品当日出荷。無料テスト機サービス。HSオールインワン蛍光顕微鏡の技術情報満載。HSオールインワン蛍光顕微鏡ならキーエンス。 BZ-9000 シリーズ (BIOREVO)HSオールインワン蛍光顕微鏡の商品情報はこちら。全商品当日出荷。無料テスト機サービス。BZ-9000 シリーズ (BIOREVO)の技術情報満載。BZ-9000 シリーズ (BIOREVO)ならキーエンス。 was compressed by manual abdominal 1:1000, ab64693, Abcam), and IL-10 (mouse IgG, 1:250, ab25073, Abcam). images were obtained with a universal fluorescence microscope (BZ9000,. Keyence). The IL Series User's Manual can be downloaded from the KEYENCE web site: „Sensor amplifier unit z DIN rail mount. A comparison between manual counts and software-assisted. 143 counts was performed in 15 images in order to validate the macro.... Images were acquired using a BIOREVO BZ-9000 slide scanner system (Keyence, Osaka, Japan). F-I: Representative images of exocrine and endocrine pancreatic CD8. mer (Agilent Technologies), X-tremeGENE HP. (Roche), and Lipofectamine 2000 (Invitrogen) were used following the manufacturers' instructions. Transfected cells were observed under a fluorescence microscope (BZ-9000; Keyence) and analyzed using. BZ-Analyzer software (Keyence). mRNA synthesis and transfection. instructions. After staining nuclei with 10 μM Hoechst 33342, cells were examined under a fluorescence microscope (model BZ-9000; Keyence). RNA isolation and semi-quantitative RT-PCR. Total RNA was isolated using RNeasy Mini kit (Qiagen) according to the manufacturer's instructions, and cDNAs were generated. (Keyence BZ-9000 Viewer); (b,c) Analysis of the viability of hyphae and microconidia of. F. solani by.. According to the manufacturer's instructions, the fusion protein was purified, and the purification. blue, stained and unstained cells were counted under the microscope (Keyence BZ-9000, KEYENCE. Although manual dissection using laser capture microscopy9 or genetic manipu- lation of ES/iPS cells has been used in many studies for isola- tion of these cells,.. Hoechst 33342 (Sigma). BrdU-positive cells were exam- ined under a Biozero fluorescence microscope BZ-9000. (Keyence). The BrdU-positive nuclei were. by reverse transfection method with Lipofectamine RNAi. MAX according to the manufacturer's instructions.. Fluorescence images were obtained by BZ-9000 (Keyence,. Osaka, Japan). Measurement of ROS. Intracellular. the Keyence BZ-9000 Analysis software. Results. Degradation of ERa protein by. The Thermoplate utilizes a special transparent heating material coated onto a hard glass. The Thermoplate can be used for observing or manipulating specimen under phase contrast and polarization. The flat plate surface ensures easy operation of the microscopes and relocation of specimen. High Quality & High. B16/F10 melanoma cells were labeled using a carboxyfluorescein diacetate cell tracker kit (Invitrogen) according to the manufacturer's instructions.. The number of adherent cells was calculated from 10 independent photographs per sample using BZ-9000E analyzer software version 1.42 (Keyence, Neu Isenburg,. For nucleus staining, cells were washed with PBS and incubated with 1.0 μg/ml 4',6-diamidino-2-phenylindole (DAPI; Carl Roth). Fluorescent microscopy images were obtained using a BZ-9000 fluorescent microscope system (Keyence, Osaka, Japan) with a 20-fold magnification and the BZ II Analyzer software (Keyence). TEX101, a germ cell-specific glycosyl-phosphatidylinositol (GPI)-anchored glycoprotein, is associated with Ly6k during spermatogenesis in testis. Although both Tex101−/− and Ly6k−/− mice can produce morphologically intact spermatozoa, both knockout mice show an infertile phenotype due to a disorder. BIOREVO BZ-9000 (KEYENCE) with CFI Plan Fluor ELWD DM 20C. (Nikon) and processed using Adobe Photoshop.. instructions. The labeled samples were hybridized to Human Promoter. Gene 1.0 ST GeneChip arrays (Affymetrix) to assess and compare overall gene-expression profiles as described. This PDF file includes: Materials and.. acquired on a Keyence BZ-9000 microscope and analyzed using ImageJ software using.. instructions. Nuclei were stained with DAPI and slides were mounted in SlowFade Gold. Antifade Reagent (Life Technologies). Image acquisition was done on a Leica SP5. In vivo cell cycle analysis in higher eukaryotes has been limited by the challenge of preserving the integrity of the living organism while visualizing dividing cells. Here, we propose a new model, which uses the unique combination of features of the Japanese medaka in order to visualize and manipulate the. Probes Inc), following the manufacturer's instructions. The stained and suspended cells were mixed and plated to form. with a BZ-9000 light microscope (Keyence). The contractibility of the cardiac spheroids and grafts was recorded using the BZ-9000 microscope imaging system. A motion analysis and measurements. the manufacturer,s instructions. Labeled cells (0.5 Â. (Keyence). The number of labeled cells was calculated using BZ-9000E analyzer software version 1.42 (Keyence). RNA Preparation and PCR... Tissue sections were photographed using a Keyence BZ-9000E microscope, and the number of positive. the company's instruction. The stained samples were observed under BIORVO microscope (BZ-9000, Keyence Japan, Osaka,. Japan). Seven images were randomly selected to calculate BrdU- positive nuclei number in each sample. Cell proliferation capacity was shown as a percentage of BrdU-positive. sliced, stained, observed using an optical microscope (BZ-9000;. Keyence. were acquired using a fluorescence microscope (BZ-9000;. Keyence). Detailed procedures are described in the. Supplementary Material. Transmission Electron Microscopy. Korea) according to the manufacturer's instructions. the manufacturer instructions. Briefly, overconfluent cultures on day 14 were subcultured in 60-mm culture dishes. The cell aggregates after SA β-gal staining were ex- amined using a Biorevo BZ-9000 microscope (Keyence). Periodic acid–Schiff staining. To identify lipofuscin granules, cultured cells were stained by Periodic. Burkholderia unamae CK43B, a member of the Betaproteobacteria that was isolated from the rhizosphere of a Shorea balangeran sapling in a tropical peat swamp forest, produces neither indole nor extracellular polymeric substances associated with biofilm formation. When cultured in a modified Winogradsky's medium. Fluoro Jade C (FJC) staining was performed according to the manufacturer's instruction. [Ready-to-Dilute. Sections were photographed at 20× magnification on a KEYENCE BZ-9000 microscope. (Keyence. ISOGEN (NIPPON GENE, Tokyo, Japan) following the manufacturer's instructions. Extracted. manufacturer's instructions. (ThermoFisher. Scientific). Knockdown of TRIM24 and TRIM32 was achieved by cloning respective synthetic. microRNAs using BLOCK-iT™ Pol II miR RNAi. Expression Vector Kit and... Keyence fluorescence microscope BZ 9000, at 10x objective (Plan Apochromat, NA: 0.45). Situ Apoptosis Detection Kit (Millipore; catalog no. S7165) according to the manufacturer's instructions. Immunofluorescent images were acquired using a. Keyence BZ-9000 fluorescence microscope. For light microscopic staining of. Olig2, spinal cord sections were blocked with 3% hydrogen peroxide in. done with the Keyence BZ-9000 microscope at 20-fold magnification. Click-iT plus TUNEL. according to the manufacturer's instructions... instructions. In short, the assay provides several beads (of known size) conjugated with a specific antibody directed against distinct cytokines. By incubating with a. Images were captured using a microscope at a magnification of ×200 (model BZ-9000; Keyence) and the area of CD31-positive vessel-like structures was. and 36 hours, the absorbance of the medium in each well was assessed using cell counting kit-8 (DOJINDO) following the manufacturer's instructions. 1:1000 and biotinylated polyclonal rabbit anti-goat IgG antibody. (Dako) was detected using the Vectastain Elite ABC Kit (Vector. Laboratories, Burlingame, CA) according to the manufacturer's instructions. Signals were captured with a BZ-9000 microscope. (Keyence, Osaka, Japan) and the background correction algorithm. using a fluorescence microscope (BZ-9000; Keyence) equipped with a Plan-. Fluor ELWD 403/0.60 objective lens (Nikon). Images were recorded with the. BZ-II viewer software, and the diameters of single cells were measured using. ogies, Madison, WI) according to the manufacturer's instructions. cence microscopy with a Keyence Biorevo BZ-9000 instrument. (Osaka, Japan). The microscope was. 470/40) and Keyence TRITC (excitation: 540/25) filters. Images were recorded with the BZ-II Viewer Software and pro-.. according to manufacturer's instructions. After isolation of the RNA, about 1 μg of. cence images were acquired with the Keyence BZ-9000 Bio-. Revo microscope (Keyence, Neu-Isenburg, Germany) with filter for DAPI, FITC/Alexa Fluor 488, and TexasRed/Alexa. Fluor 568/594 and the BZ-II Viewer software (Keyence). Antibodies.. according to the manufacturer's instructions (LSBio, Seattle,. WA, USA). BZ-9000 fluorescent microscope system (Keyence,. Osaka, Japan). 2. BMJ Open Diabetes Research and. cells (7.16±0.59 μm), using the BZ 9000 fluorescent microscope system.17. 18. In addition, cell death was. proliferation assay (Roche Diagnostics, Ltd, Burgess. Hill, UK) according to the manufacturer's instructions. Frozen sections of hCE alone (day 0) and of wound specimens from the hCE group (day 7) were observed under a fluorescence microscope (KEYENCE BZ-9000 and BZ-II Analyzer ver. 1.42; KEYENCE Japan, Osaka, Japan) using a tetramethylrhodamine isothiocyanate filter. For the comparison, the sections were stained. labeling was conducted according to the manufacturer's instructions. The stained cells were visualized using a flu- orescence microscope (BZ-9000, Keyence). PI-positive cells were detected with a fluorescence filter for Texas. Red and autophagosomes were detected with a UV filter. Statistical analysis. facturer's instructions. Fluorescence was visu- alized with a fluorescence microscope (BZ-. 9000; Keyence, Osaka, Japan). To assess the annexin V-positive apoptotic cells, 300 cells were counted in a given microscopic field. The results were obtained from three independent experiments. Evaluation of. Cancer stem cells (CSCs) are generally dormant or slowly cycling tumor cells that have the ability to recon- stitute tumors. They are thought to be involved in tumor resistance to chemo/radiation therapy and tumor relapse and progression. However, neither their existence nor their identity within many cancers has been well. Affinity Gel following the manufacturer's instructions. Empty vector.. 6 C. Images were captured on Keyence microscope, and cell surface area was measured using MacroCellCount analyzer as detailed in the Ma-... Immunofluorescence pictures were taken using the BZ-9000 microscope. For each. Tissue sections were examined with the Keyence BZ-9000 microscope equipped with a BZ-. 9000 Analyser. taken at 20x objective with BZ-9000 Keyence microscope and analysis performed with BZ-II. Analyser Software. according to the manufacturer´s instructions (PeqLab, 30-2040). It was treated with. Pictures were taken with the microscope software (Keyence BZ-9000 Viewer); (b,c) Analysis of the viability of hyphae and microconidia of F. solani by performing the.. According to the manufacturer's instructions, the fusion protein was purified, and the purification steps were analyzed via SDS-PAGE. with a BZ-9000 fluorescence microscope (KEYENCE). Heparinase digestion and detection of. were mounted with Fluoromount and analyzed with a BZ-9000 fluo- rescence microscope, as described. facturer's instructions (TaKaRa Thermal Cycler Dice TP870). The primer sets used were as follows:.
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