Saturday 17 February 2018 photo 4/15
|
Southern blotting protocol pdf: >> http://hip.cloudz.pw/download?file=southern+blotting+protocol+pdf << (Download)
Southern blotting protocol pdf: >> http://hip.cloudz.pw/read?file=southern+blotting+protocol+pdf << (Read Online)
southern blot hybridization buffer
southern blot plasmid dna
southern blot steps
southern blotting protocol method
southern blot troubleshooting
southern blot protocol non-radioactive
southern blot negative control
southern blot hybridization protocol
Southern Blot Protocol – Gause Laboratory. Page 1 of 1. SOUTHERN BLOT PROTOCOL. Used by the Laboratory of William C. Gause, Ph.D. BUFFERS. 10X TBE: (1 Liter). 20X SSPE: (4 Liters). TRIS Base. 108.0 g. NaCl. 701.2 g. Boric Acid. 55.0 g. NaH2PO4 H2O 110.4 g. *Dissolve reagents in 3200mL. 0.5 M EDTA 40.0
K. Maxson-Stein modified from Hui's 2010 version. Date Modified: 2/11/15. Research:Brutnell_labKimSouthernsNew Southern Protocol Feb 2014 w Depurination.doc. SOUTHERN BLOT PROTOCOL. Genomic DNA Digestion: Reagents. Vol. Reagents. Vol. gDNA (10ug). 20.7 ?l. gDNA (10ug). 20.7 ?l. Buffer (10x). 2.5 ?l.
Southern-blot Analysis. Protocol. I. Salt transfer. - Cut membrane (GeneScreen Plus, NEN Research Products, Cat# NEF-976) to size of gel. - Wet membrane by placing in a tray containing distilled water. - Transfer to a tray containing 10X SSC and allow to equilibrate for at least 15 minutes. - Agitate gel in a tray containing a
Department of Agronomy. Southern Blot. 1. Updated 03-14-05. Southern Blotting. Materials. ? Depurination Solution. 200 mM HCl. ? Denaturation Solution (1L). 1 M NaCl (58.4 g). 0.5 M NaOH (20 g). ? Neutralization Solution (1L). 3 M NaCl (175.3 g). 0.5 M Tris (60.6 g). pH to 7.4 with NaOH or HCl before voluming to 1L.
SOUTHERN BLOTTING ONTO A NYLON OR NITROCELLULOSE. MEMBRANE WITH HIGH-SALT BUFFER. The procedure is specifically designed for blotting an agarose gel onto an uncharged or positively charged nylon membrane. With minor modifications, the same protocol can also be used with nitrocellulose
Southern Blotting and. Related DNA Detection. Techniques. Terence A Brown,University of Manchester Institute of Science and Technology, Manchester, UK. Southern blotting is a technique for transfer of DNA molecules from an electrophoresis gel to a nitrocellulose or nylon membrane, and is carried out prior to detection
c ol. Southern Blotting. Dot Blotting. Required solutions. 1. Denaturation solution: 1.5 M NaCl, 0.5 M NaOH. 2. Neutralization solution: 1.5 M NaCl, 0.5 M Tris- 50 ng of marker is sufficient for generation of radioactively labeled probe for 3-5 hybridization reactions. This protocol is for the Southern Blotting. Dot Blotting
probe. The properties and advantages of the different membranes are discussed more fully in the commentary. The basic protocol describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon or nitrocellulose membrane, using a high-salt transfer buffer to promote binding of DNA to the
Southern Blotting. MSU Potato Lab. A. DNA AGAROSE GEL. 1. Prepare a 1% agarose gel and load 5 ?l of BMB Molecular Weight Marker DIG labeled in one lane. 2. Mix 20?g Plant Genome DNA (which has been digested with an .. more (this will give you some to spare) of the gene using the PCR protocols described for.
Southern Blot: - turn the gel over and put on clean table (gloves!) - wet a piece of nylon membrane of the size of the gel in neutralizing solution and lay it onto the gel (no airbubbles!) - wet 3 pieces of 3mm Whatman paper (same size as gel!) in neutralizing solution and lay onto gel, one after each other (no airbubbles!)
Annons