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Pgem t easy manual promega corporation: >> http://rux.cloudz.pw/download?file=pgem+t+easy+manual+promega+corporation << (Download)
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PCR Cloning with Blue/White Selection and Easy Insert Excision. The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. Complete Protocol. PDF Download Icon. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual. PDF (434 KB)
The sequence of the pGEM®-T Easy Vector is available at: www.promega.com/vectors/. The pGEM®-T Easy Vector has been linearized at base 60 with EcoRV and a T added to both 3?-ends. The EcoRV site will not be recovered upon ligation of the vector and insert. Promega Corporation · 2800 Woods Hollow Road
Ligation Using 2X Rapid Ligation Buffer. 1. Briefly centrifuge the pGEM®-T or pGEM®-T Easy Vector and Control Insert. DNA tubes to collect contents at the bottom of the tube. 2. Set up ligation reactions as described below. Vortex the 2X Rapid Ligation. Buffer vigorously before each use. Use 0.5ml tubes known to have low
The pGEM®-T and pGEM®-T Easy Vector Systems are convenient systems for the cloning of PCR products. The vectors are prepared by cutting the pGEM®-5Zf(+) and pGEM®-T Easy Vectors, respectively, with EcoR V and adding a 3? terminal thymidine to both ends. The pGEM®-T and pGEM
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Telephone 608-274-4330 · Fax 608-277-2601 · www.promega.com. Printed in USA. Part# TM042. Revised 11/98. pGEM®-T and pGEM®-T. Easy Vector Systems. INSTRUCTIONS FOR USE OF PRODUCTS
4,766,072 has been issued to Promega Corporation for transcription vectors having two different bacteriophage RNA polymerase promoter sequences separated by a series of unique restriction sites into which foreign DNA can be inserted. The high copy number pGEM®-T and pGEM®-T Easy Vectors contain T7 and SP6
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Telephone 608-274-4330 · Fax 608-277-2516 · www.promega.com. Part# TM042. Printed in USA. Revised 3/03. I. Description. The pGEM®-T and pGEM®-T Easy Vector Systems(a,b) are convenient
Cloning - Promega pGEM-T Easy kit. Ligation. 1. Add to the reaction mixture: a. 3 µl Fresh PCR product b. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase. 2. Incubate at room temperature for 1 hour or overnight at 4°C. 3. Store on ice until needed. Transformation. 1. Get cells out of freezer, thaw on
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516. TM042 · Revised 6/15 www.promega.com. 3. Protocol for Ligations Using the pGEM®-T and pGEM®-T Easy Vectors and the 2X Rapid Ligation. Buffer. 3.A. Ligation
PCR products were cloned using the pGEM®-T Easy Vector System and the LigaFast™ Rapid DNA Ligation System. Rosalia Sirchia, Valentina Ciacciofera and Recombinant plasmids were purified using a commercial miniprep kit and quantitated by gel electrophoresis. Insertion of the PCR product of interest into the
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