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Antarctic phosphatase neb manual: >> http://fnh.cloudz.pw/download?file=antarctic+phosphatase+neb+manual << (Download)
Antarctic phosphatase neb manual: >> http://fnh.cloudz.pw/read?file=antarctic+phosphatase+neb+manual << (Read Online)
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Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5? and 3? ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in
3 Oct 2014 This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289). New England Biolabs (NEB). 1. Add 1/10 volume of 10X Antarctic Phosphatase Reaction Buffer to 1-5 µg of DNA cut with any restriction endonuclease in any buffer. 2. Add 1 µl of Antarctic
Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzy.
WWW: www.neb.com. Technical Bulletin. #M0289 (9/19/04). #M0289S 1,000 units . . . . . . . $58 (USA). #M0289L 5,000 units . . . . . . $232 (USA). Description. Antarctic Phosphatase catalyzes the removal of 5? phosphate groups from DNA and RNA. Ligation Kit (NEB #M2200). Vector Dephosphorylation Protocol: 1.
This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289).
lated in restriction endonuclease buffer supple- mented with Antarctic Phosphatase Reaction Buffer. Ligation is performed with 50 ng of vector using the. NEB Quick Ligation Kit (NEB #M2200). Heat Inactivation: 70°C for 5 minutes. Protocol for Dephosphorylation of 5?-ends of. DNA using Antarctic Phosphatase. 1. Prepare a
NEB Quick Ligation Kit (NEB #M2200). Vector Dephosphorylation Protocol: 1. Add 1/10 volume of 10X Antarctic Phosphatase. Reaction Buffer to 1– 5 µg of DNA cut with any restriction endonuclease in any buffer. 2. Add 1 µl of Antarctic Phosphatase (5 units) and mix. 3. Incubate for 15 minutes at 37°C for 5? extensions.
Protocol for Dephosphorylation of 5?-ends of DNA using Antarctic Phosphatase (NEB #M0289). Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protocol. Prepare a 20 ?l reaction as follows:
Advantages and Features. Applications. Dephosphorylation 5? and 3? ends of DNA and RNA. Dephosphorylation of cloning vector DNA to prevent recircularization during ligation. Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase. Treatment of dNTPs in PCR reactions prior to sequencing or
"Antarctic Phosphatase catalyzes the dephosphorylation of 5? and 3? ends of DNA and RNA phosphomonoesters. Antarctic Phosphatase also hydrolyzes ribo-, as well as Antarctic Phosphatase. New! Quick Dephosphorylation Kit offers heat inactivation, high specific activity and a fast protocol. $name heat inactivation.
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