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Strataclone pcr cloning kit manuals: >> http://aal.cloudz.pw/download?file=strataclone+pcr+cloning+kit+manuals << (Download)
Strataclone pcr cloning kit manuals: >> http://aal.cloudz.pw/read?file=strataclone+pcr+cloning+kit+manuals << (Read Online)
Features DNA topoisomerase I and Cre recombinase technologies; Blunt-end and UA PCR cloning kits now available; Clone both long and short amplicons with the same kit; High efficiency yields >95% insert positive clones; Simple, three step protocol with 5-minute room temperature ligation; StrataClone Ultra™ PCR
High efficiency UA PCR cloning, features DNA topoisomerase I and Cre recombinase technologies 20 reactions Description, VWR Catalog Number, Supplier No, Unit, Price, Quantity. KIT STRATACLONE PCR CLONING 20, 99901-042, 240205, Each, Retrieving
16 Nov 2012 The Strataclone PCR Cloning Kit seems to be one of those, fulfilling almost all of the researcher's needs. It is a robust kit In conclusion, this cloning kit is highly recommended for its time savings, its double antibiotic site for cell antibiotic resistance (amp/kan) and the simple and easy to follow handbook.
Sencillo y eficiente clonaje de amplicones cortos y largos. > 95% de clones con inserto. -, Protocolo corto de 3 pasos, 5 minutos de ligacion a temperatura ambiente. -, Celulas competentes StrataCloneTM SoloPack® incluidas. -, No requiere primers de PCR con disenos especiales. -, No requiere modificaciones post-PCR
Even if the cloning of the insert didn't work you should see blue colonies. This means (a) something is wrong with your cells. Did you do a control transformation with the provided pUC18 plasmid? Or (b) something is wrong with your plates. By the way, according to the manual you should use 5–50 ng of your PCR product
This warranty limits our liability to replacement of this product. No other warranties of any kind, express or implied, including without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by Agilent. Agilent shall have no liability for any direct, indirect, consequential, or incidental
from topoisomerase I-mediated ligation of two linear StrataClone vector arms to a PCR product of interest, followed by Cre- mediated recombination. Please see the StrataClone™ PCR Cloning Kit instruction manual for additional information. kanamycin ampicillin f1 ori. <loxP>. pUC ori. P lac. MCS. PCR Product Insertion
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature.
This warranty limits our liability to replacement of this product. No other warranties of any kind, express or implied, including without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by Agilent. Agilent shall have no liability for any direct, indirect, consequential, or incidental
Thermo Scientific CloneJET PCR Cloning Kit is an advanced positive selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any other blunt or sticky-end DNA fragment can be cloned. It is ideal for phosphorylated or non-phosphorylated DNA fragments.
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