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Tmre protocol: >> http://bit.ly/2ySrCOe << (download)
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Overview of the MitoPT TMRE/TMRM Protocol 7. 8.Induction of Apoptosis or Mitochondrial Depolarization .7. 9.Preparation of 1X
Cayman's TMRE Mitochondrial Membrane Potential Assay Kit utilizes . TMRE for 30 minutes as described in the assay protocol. (A) Saturation of signal.
Figure 1. Flow cytometric analysis of Jurkat cells, unlabeled (black) or labeled with 200 nM TMRE and treated with 0 ?M CCCP (blue), 3.2 ?M CCCP (green),
MitoStatus TMRE (Tetramethylrhodamine ethyl ester) has an excitation . Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Protocol for measuring mitochondrial potential has also been included in this chapter. In conclusion .. Stain with TMRE as in Support Protocol 2. Repeat step 1
TMRE emits a red fluorescence that can be detected by flow cytometry or Treat the cells with a cytotoxic stimulus as described in Protocol: Triggering
Negative Control (FCCP, 40 mM): Aliquot and store at -20°C. Thaw at RT. VII. TMRE Mitochondrial Membrane Potential Assay protocol: 1. Sample Preparation:.
Tetramethylrhodamine, ethyl ester (TMRE) is a cell-permeant, cationic, red-orange fluorescent dye that is readily sequestered by active mitochondria.
TMRE-Mitochondrial Membrane Potential Assay (ab113852). Measure instructions. Store at +4°C. Please refer to protocols. Protocols. Protocol Booklet
30 Mar 2015 Abcam's TMRE Mitochondrial Membrane Potential Assay Kit is designed for . protocol completely to confirm this kit meets your requirements.
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