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Page 1. tac promoter lac O. RBS prescission protease. pGEX-6P-1. 5.Okb. BamH I. EcoRI. Smal. Sal |. Xho |. Not I. T.
Amersham Pharmacia Biotech. pGEX Vectors(GST Gene fusion). All of the GST gene fusion vectors offer: • A tac promoter for chemically inducible, high-level expression. Map of the glutathione S-transferase fusion vectors showing the reading frames and main features. Even though stop codons in all three frames are not
consists of three major components: pGEX plasmid vectors, two GST Purification pGEX Vectors*. All of the GST gene fusion vectors offer: s A tac promoter for chemically inducible, high-level expression. s An internal lac Iq gene for use in any E. coli host. Resuspend bead in 25 µl of water per standard instructions. 2.
The system consists of three major components: pGEX plasmid vectors, products for. GST purification, and GST detection products. A series of site-specific proteases for GST-tag cleavage complements the system. The pGEX vectors are designed for inducible, high-level intracellular expression of genes or gene fragments
expression vector. pGEX vectors. GST fusion proteins are constructed by inserting a gene or gene fragment into the multiple cloning site of one of the ten pGEX vectors. Expression is under the control of the tac promoter, which is induced by the lactose analog isopropyl b-D thiogalactoside (IPTG). All. pGEX vectors are also
The objective of our work was to over express GST protein, from PGEX 3X in the BL21 strain of Escherichia coli. The GST protein was induced with 0.1mM of PGEX 3X vector was isolated by alkali lysis method. The following solution were used .. A Laboratory Manual, Vol. 1, 2 and 3. 3rd edition.,. Cold Spring Harbor
thrombin or factor Xa protease sites to cleave protein from fusion. pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs. Hosts: E.coli. Related vectors: pGEX-2T. (Information source: VectorDB.) Catalog Number: 27458001.
pGEX-4T-1. GST Expression Vector. Product Specification Sheet. Code: 28-9545-49. Warning. For research use only. Not recommended or intended for diagnosis of disease in humans or For manual purification of sample volumes up to 600 µl use GST For more information on the use of pGEX vectors, see GST Gene.
The production of soluble, intact GST fusion pro- teins requires optimization of several factors. This discussion focuses on choosing the proper host strain, growth temperature, cell density at time of induction and length of induction for expression of a GST- luciferase fusion protein. Examples demonstrate that for this fusion
Several pGEX vectors are available with multiple cloning sites to allow for unidirectional insertion of the coding-region DNA into the pGEX vector. The GST fusion protein is easily purified by affinity chromatography using a glutathione-Sepharose matrix under mild conditions. Removal of the GST moiety from the protein of

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March 2018