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11 Oct 2016 Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at specific sequences. For a list of many commonly used restriction enzymes, visit NEB. Restriction enzyme digestion is commonly used in molecular cloning techniques, such as PCR or
7 Dec 2012 Standard Protocol for Restriction Enzyme Digests This enzyme : DNA : reaction volume ratio can be used as a guide when designing
Restriction enzyme digestion of DNA (basic method). A shared scientific protocol. Share your scientific methods at methodbook.com.
Setting up a Restriction Enzyme Digestion . Additional Protocols for Selected Restriction Enzymes . A. Protocol for Rapid Digestion of Plasmid DNA .
Introduction. Restriction enzymes, also referred to as restriction endonucleases, are enzymes which recognize short, specific (often palindromic) DNA
Protocols for use of Promega Restriction Enzymes, including basic information on reaction setup and controls. Protocols for rapid digestion of plasmid DNA in
DNA double digestion protocol. Materials: 0 DNA sample(s) in water or TE buffer. 0 10x digestion buffer. 0 Restriction enzymes (EcoRI or SpeI or XbaI or PstI).
Prepare negative control reaction without template DNA. Prepare positive control reaction with template of known cutting site corresponding to the restriction
2= Restriction enzyme activity is measured in “units." One unit is defined as the amount of the enzyme required to digest 1 ug of DNA in 60 minutes. 10-fold
Sigma® Life Science's restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use

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August 2017