Sunday 19 November 2017 photo 11/15
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Gibco Sf9 cells are adapted to serum-free suspension culture in Gibco Sf-900 II SFM, which User Guide: Sf9 Cells Adapted in Sf-900 II SFM and Sf-900 III SFM.
23 Aug 2017 Spodoptera frugiperda (Sf9, Invitrogen) cells were cultured in SF-900 medium reagent (Invitrogen) following the manufacturer's instructions.
Ex-Cell 302 has also been used successfully with BHK-21 cells and chicken Sf9) as the host cell of choice for infection with recombinant Autographa.
Sf9 Insect Cells and BacVector® Insect Cell Medium (pages 14,15). For optimized T7 terminator. Chicken ?-actin TriEx™ Insect Cell Medium (page 15) • Gibco® Sf-900 II SFM. • ESF 921 according to the standard protocol . Protein yield
Lepidopteran insect cell culture technology has progressed to the point of becoming an essential part of one of the most successful eukaryotic expression
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14. Technical Protocols. Storage and Growth of Sf9 and TriEx™ Sf9 Insect Cells . . T7 terminator. Chicken ?-actin TriEx™ Insect Cell Medium (page 15) • Gibco® Sf-900 II SFM. • ESF 921 according to the standard protocol . Protein yield,.
10 Jun 2017 Primatone RL, a meat hydrolysate, has been replaced by soy protein hydrolysate to commercial supplier (e.g., High Five cells from Invitrogen have been . spinner culture protocol for S. frugiperda, exponential-phase monolayer sion in serum-free cultures of Sf9 insect cells: modulation by conditioned
Manuals · Supplemental Protocols Optimized to support Sf9 and Sf21 cells — achieves Sf9 densities of 10-14 X106 cells/ml. Experience the difference with Sf-900™ III SFM. Gibco® Sf-900™ III SFM is specifically designed to offer high performance Sf9 cell growth in Sf-900™ III SFM compared to other insect cell culture
TubeSpin bioreactor 50 for the high-density cultivation of Sf9 insect cells in suspension .. manual counting using the Trypan Blue exclusion method. .. Sf-9 insect cells were routinely cultivated in 5 mL of Sf900 II SFM medium (Invitrogen, . screen for seven potential substitutes for the default meat peptone, Primatone RL.
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