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Tmrm protocol: >> http://bit.ly/2x9ON9w << (download)
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This protocol describes a high-content microscopy approach to quantifying mitochondrial morphofunction in living cells. After staining with fluorescent reporters
Label mitochondria in live or fixed cells using Manuals & Protocols. Manuals; TMRM and TMRE have been used to measure mitochondrial depolarization
Protocols using MitoPT TMRE or TMRM at concentrations > 50 nM should generally include a single wash step to minimize background fluorescence issues.
dyes are well-retained following fixation and permeabilization using the protocol described below. However,
Loss of mitochondrial membrane potential can be detected by a unique The MitoPT TM TMRM reagent easily penetrates cells For general protocol
Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from
The co-localization of MitoTraker Green and TMRM in polarized mitochondria allows a fluorescence resonance energy protocols do not require tricks to
Technical Data Sheet MitoStatus TMRE Product Information Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols. References
Where [TMRM] t is the total mitochondrial accumulation (in units of nmol/mg), K i and K o are the partition coefficients (in units of ?l/mg), V im and V m are the
Analysis of Mitochondrial Membrane Potential Please suggest any paper or protocol to accomplish that. TMRM definitely outperforms mitotracker for time lapse
Analysis of Mitochondrial Membrane Potential Please suggest any paper or protocol to accomplish that. TMRM definitely outperforms mitotracker for time lapse
Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader total TMRM fluorescence minus the CCCP treated TMRM fluorescence.
MitoPT™ JC-1, TMRE and TMRM apoptosis kits; mitochondrial membrane potential detection kits to distinguish apoptotic from non-apoptotic cells; flow and IF detection
assess mitochondrial membrane potential with Mitopt.TM and MitoPTTM-TMRM. prices and protocols available at www.immunochemistry.com
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