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18 Mar 2014 Gene silencing and gene expression in phytopathogenic fungi using a plant virus vector. Tiziana Masciaa,b,1, Franco Nigroa, Ali Abdallaha, Massimo Ferraraa, Angelo De Stradisb, Roberto Faeddac,. Peter Palukaitisd,1, and Donato Gallitellia,b. aDipartimento di Scienze del Suolo, della Pianta e degli
Overexpression of chimeric transgenes in plants can trigger post-transcriptional gene silencing that is dependent on epigenetic information and physiological conditions. The current view is that unproductive RNA serves as a crucial signal for gene silencing, although direct evidence is lacking for this theory. A signalling
OVEREXPRESSION/SILENCING OF. SELECTED SOYBEAN GENES ALTERS. RESISTANCE TO PATHOGENS. Mohamed H. El-Habbak. University of Kentucky, m.elhabbak@uky.edu. This Doctoral Dissertation is brought to you for free and open access by the Plant Pathology at UKnowledge. It has been accepted for
virus-resistant plants. A major goal of current research is to dissect the mechanism(s) of these sequence-homology- dependent gene silencing phenomena. Various factors seem to play a role, including DNA methylation, transgene copy number and the repetitiveness of the transgene insert, transgene expression level,
Summary. Endeavours to obtain elevated and prolonged levels of foreign gene expression in plants are often hampered by the onset of RNA silencing that negatively affects target gene expression. Plant virus–encoded suppressors of RNA silencing are useful tools for counteracting silencing but their wide applicability in
Gene silencing is the regulation of gene expression in a cell to prevent the expression of a certain gene. Gene silencing can occur during either transcription or translation and is often used in research. In particular, methods used to silence genes are being increasingly used to produce therapeutics to combat cancer and
ABSTRACT: RNA interference, transcriptional gene silencing, virus induced gene silencing, and micro RNAs comprise a series of mechanisms capable of suppressing gene expression in plants. These mechanisms reveal similar biochemical pathways and appear to be related in several levels. The ability to manipulate
Transgene-induced post-transcriptional gene silencing (PTGS) has been detected in plants (van der Krol et al., 1990) where it is called co-suppression, and in . it would explain why ectopic expression of p25 inhibits local PTGS induced by transgene but not by replicating virus (Voinnet et al., 2000), while still inhibiting the
16 Jul 2016 Co-Suppression. Download PDF (6,857 KB). In co-suppression, when a gene is introduced by transformation into a cell, neither the resident nor the transgene copy of the same gene is expressed (repeat-induced gene silencing) or increasing the gene copy number reduces the degree of expression.
expression. Has been used to. Copy number of DNA insertions can be high and lead to produce transplastomic plants. No gene silencing/cosuppression. plant host range problems. Electroporation. Very effective for transient. Requires the use of a tissue culture regeneration procedure. expression. No plant host range.
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