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17 Nov 2012 This method stains pollen grains for an enzyme as the vital indicator of membrane integrity. Only fertile grains fluoresce under microscopic
1.3.1 Development of the flower in Campanulaceae. 1.3.2.2.1 In vitro germination for pollen viability. .. 3.3.2 Results of the pollen tube germination test . . Table 3.9 Total amount of protein according to the Bradford method. 94. Table 3.10
of pollen tube growth may be employed as a measure of fertilizing potential. In vitro germination tests have been the most popular method of testing pollen
Abstract We tested pollen viability of eight species us- ing four vital dyes, a new peroxidase test together with three other established methods (MTT, Baker's and.
viability test which is quick, simple, and reliable. Treating the This is the most commonly used test for pollen viability. For the protocol of the in vitro germination test, d. gradation in color development (from very light to deep red); therefore.
The choice of method depends on the crop or species (Dafni and Firmage, 2000; (2) Which staining technique is reliable for testing J. curcas pollen viability?;
Methods for testing pollen viability in rice were evaluated by comparing staining reasons for the sensitivity at this stage of development were not evaluated.
1 Sep 2016 Dupl'akova et al. describe a protocol to separate different developmental stages of the male gametophyte (MG) in A. thaliana. To achieve this
18 Sep 2014 The IKI and TTC staining tests and pollen germination had low Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability speed of germination of pollen grains and the rate of pollen tube growth [5]. important to use a suitable method for determining pollen viability [7].
was to evaluate the pollen viability of six Passiflora species collected from different . and the means were compared using Tukey's test at growth. Each species requires a specific protocol of culture medium to obtain adequate pollen grain.
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