Thursday 22 March 2018 photo 13/15
![]() ![]() ![]() |
Nextera low-plex pooling guidelines: >> http://dts.cloudz.pw/download?file=nextera+low-plex+pooling+guidelines << (Download)
Nextera low-plex pooling guidelines: >> http://dts.cloudz.pw/read?file=nextera+low-plex+pooling+guidelines << (Read Online)
Nextera DNA Sample Preparation Workflow. 18. Tagmentation of Genomic DNA. 19. Clean-Up of Tagmented DNA. 21. PCR Amplification. 23. PCR Clean-Up. 29. Validate Library. 32. Pool Libraries. 33. Dual Indexing Principle. 36. Low Plexity Pooling Guidelines. 37. Technical Assistance. ILLUMINA PROPRIETARY.
This document provides important guidelines for low-plex pooling with Nextera Rapid Capture Enrichment. If you choose the dual index sequencing workflow for Nextera Rapid Capture Enrichment, always use at least two unique and compatible barcodes for each index (index. 1 and index 2). Each Nextera Rapid Capture
Pooling Guide. Introduction. 3. Pooling Guidelines. 3. Four-Channel Sequencing. 3. Two-Channel Sequencing. 4. TruSeq Pooling Guidelines. 4. Nextera Pooling Guidelines. 12. Next Steps. 13. Technical Follow these low-plex pooling guidelines, depending on your index adapter component. Not all color-balanced pools
Index adapter name, the N refers to Nextera library preparation, 7 or. 5 refers to Index 1 (i7) or Index 2 (i5), respectively, and 01–12 refers to the Index number. A list of index sequences is provided for generating sample sheets to demultiplex the samples (Table 1). Nextera Low Plex Pooling Guidelines. Table 1: Sequences
C h a p ter. 2. Nextera RapidCapture Guide. 3. Chapter 2 Getting Started. Getting Started. Introduction. 4. Acronyms. 5. Kit Contents. 7. Consumables and Equipment. 19. Tracking Tools. 23. DNA Input Recommendations. 25. Best Practices. 26. Dual Indexing Principle. 31. Low-Plex Pooling Guidelines. 32
Low-Plex Sample Pooling: Therefore, in order to assure that indexes of low-plex lanes (2-4 unique indexes) are accurately sequenced it is crucial to select adapter combinations that result in each laser reading a base during Keep these rules in mind when selecting indexes for low-plex pooling (2-4 libraries per lane).
Index adapter name, the N refers to Nextera sample preparation, 7 or. 5 refers to Index 1 (i7) or Index 2 (i5), respectively, and 01–12 refers to the Index number. A list of index sequences is provided for generating sample sheets to demultiplex the samples (Table 1). Nextera Low Plex Pooling Guidelines. Table 1: Sequences
19 Oct 2017 I'd be using Nextera XT DNA sample preparation index kit and MiSeq v2 reagent kit. If I select index pairs with maximum diversity, might it be safe to try? What would And here is the link for Nextera low plex pooling guide: https://www.illumina.com/documents/pguidelines.pdf. nucacidhunter is offline
25. PCR Clean-Up. 30. Library Normalization. 34. Library Pooling and MiSeq Sample Loading. 39. Clustering Nextera XT Samples for HiSeq, HiS-. canSQ, and GAIIx. 42. Dual Indexing Principle. 44. Low Plexity Pooling Guidelines. 45. Technical Assistance. ILLUMINA PROPRIETARY. Part# 15031942 Rev. C. October 2012
This document provides important guidelines for low-plex pooling with Sequencing Workflow: Single Index. Plexity. Index 1 (i7) Selection. Index 2 (i5). Selection. 1 plex (no pooling) Any Index 1 adapter. Any Index 2 adapter. 2 plex Illumina, HiSeq, MiSeq, Nextera, NextSeq, TruSeq, and the pumpkin orange color are.
Annons