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DOWNLOAD X gal screening protocol for cochlear: >> http://bit.ly/2wDEaLF <<
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In the cochlea, the mammalian auditory organ, fibrocytes of the mesenchymal . X-Gal staining and histological analysis. . The care and use of animals followed the animal welfare guidelines of the Institut National de la Sante A ? phage containing the entire mouse Otos gene was isolated by screening a ? FIX II genomic
coding region were isolated by screening a 129Sv/J mouse genomic library (Stratagene) conjugated phalloidin following the manufacturer's protocol. (Molecular deletion, lacZ expression was detected in hair cells of the organ of Corti as
19 Nov 2009 A limiting factor of X-gal staining is the low contrast to e.g. blood-related . a novel protocol to isolate LacZ-expressing cells from the neonatal cochlea. .. for the screening of drugs and toxicants and demonstrate its use here.
Plates for Blue/White Colony Screening Add 40 ?L of the Thermo Scientific X-Gal Solution (20 mg/mL), ready-to-use (Cat #R0941). 4. This protocol is for the Preparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening.
5 Aug 2016 In the developing cochlea, Wnt/?-catenin signaling positively regulates the .. Using this protocol, we stained cochleae from 3 Lgr4-LacZ knock-out The width of the screen corresponded to 150 ?m of the specimen and the
25 Mar 2009 Although outer hair cells (OHCs) play a key role in cochlear amplification, Using the membrane-based yeast two-hybrid system to screen a newly built After incubating at 30°C for 2–3 days, the X-Gal staining assay was performed kidney (OK) cells according to the protocols described in Zheng et al.
Prepare 20 mg/ml X-Gal solution in DMF (See X-Gal Stock Solution Procedure). We recommend using a higher concentration of X-Gal than most protocols.
8 Feb 2016 Wbp2 mutation and Wbp2 expression in the cochlea X?Gal staining at post?natal day (P) 14 showed the expression of Wbp2 in all in a broad phenotypic screen, other than reduced circulating amylase levels in .. Experiments and analysis were carried out in compliance with the ARRIVE guidelines.
2 Nov 2012 Singly isolated cochlear inner and outer hair cells express HCN1 transcript, and and protein-protein interaction protocols have now yielded results consistent Yeast was plated on quadruple dropout media containing X-?-Gal and The co-transformation screening was performed once, and then the
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