Saturday 17 February 2018 photo 2/15
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Installing MUSCLE · Making an alignment (MSA) · Creating a tree (N-J or UPGMA) · License: MUSCLE is public domain. MUSCLE papers, how to cite. Papers and citing MUSCLE Popular options and features. Alignment output formats · Adding a sequence to an existing MSA · Profile-profile alignment (aligning two MSAs)
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If muscle completes successfully, it will create a file seqs.afa containing the alignment. By default, output is created in "aligned FASTA" format (hence the .afa extension). This is just like regular FASTA except that gaps are added in order to align the sequences. This is a nice format for computers but not very readable for
We remind you that it is not long until the EBI's Wise2DBA and Promoterwise services are retired on 15th April 2018. MUSCLE stands for MUltiple Sequence Comparison by Log- Expectation. Important note: This tool can align up to 500 sequences or a maximum file size of 1 MB.
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