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31 Oct 2017 Meanwhile, if you receive 0.5nM, look at the protocol, you could do as below: Starting Library Concentration Library 0.2 N NaOH 4 nM 5 µl 5 µl 2 nM 10 µl 10 µl 1 nM 20 µl 20 µl 0.5 nM 40 µl 40 µ. The protocol: NextSeq System Denature and Dilute Libraries Guide Document # 15048776 v02. GA-J is offline
Document about Nextseq 500 Sequencing System University Of Connecticut is available on print and digital sequencing consumables overview nextseq 500 system guide (15046563) illumina instructions for using the transcriptome nextseq system denature and dilute libraries guide (15048776) - nextseq system.
13 Oct 2016 I am following the Nextseq System-denature and dilute libraries guide. The lowest starting library concentration recommended for denature libraries is 0.5 nM. However, the conc. of one of my libraries is particularly low and the final volume would be more than 40 ul. I would like to know if the volume of the
Document# 15048776 v02. January2016. NextSeqSystem. Denature andDilute Libraries Guide. ForResearchUse Only. Not foruse indiagnosticprocedures. Overview. 3. Protocol A: Standard Normalization Method. 5. Protocol B: Bead-Based Normalization Method. 7. Denature and Dilute PhiX Control. 8. Next Steps. 10.
For best results, begin thawing the reagent cartridge before denaturing and diluting libraries. For instructions, see the MiniSeq System Guide (document # 1000000002695). Document # 1000000002697 v00 Use protocol A to denature and dilute libraries that have been normalized using standard library quantification and
Part# 15048776 Rev. A. January2014 the NextSeq™ 500. FORRESEARCH USEONLY. Introduction. 3. Prepare Reagents. 4. Denature and Dilute Libraries. 5. Denature and Dilute PhiX Control. 7 This document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"), and are intended solely for the.
29 Nov 2017 Note that both these loading quantities are higher than that typically recommended by Illumina, which is 1.8 pM (NextSeq System Denature and Di- lute Libraries Guide, Document #15048776, January 2016). Furthermore, we have found that for single-stranded libraries, cluster densities can be raised
Denaturing and Diluting Libraries for the NextSeq System (document. # 15048776). Provides instructions for denaturing and diluting prepared libraries for a sequencing run, and preparing an optional PhiX control. This step applies to most library types. NextSeq Custom Primers Guide. (document # 15057456). Provides
14 Nov 2016 This document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"), and are intended solely for the This guide explains steps to denature and dilute libraries after sample preparation to prepare Always prepare freshly diluted NaOH for denaturing libraries for cluster generation.
This guide contains instructions for denaturing and diluting libraries after library preparation and before sequencing on the NextSeq System. This guide also includes instructions for preparing a PhiX control. Files. File Name. File Info. Date Posted. NextSeq System Denature and Dilute Libraries Guide (15048776 v02) PDF

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March 2018