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Getting started. Installing MUSCLE · Making an alignment (MSA) · Creating a tree (N-J or UPGMA) · License: MUSCLE is public domain. MUSCLE papers, how to cite. Papers and citing MUSCLE Popular options and features. Alignment output formats · Adding a sequence to an existing MSA · Profile-profile alignment
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Articular surfaces. Atlanto-occipital joint: convex (occipital condyles of occiput) on concave (superior articular facets of atlas). Atlantoaxial: concave (articular facet for dens on atlas) on convex (anterior facet of dens on axis). Zygapophyseal: facets are oriented 45°. Intervertebral discs: horizontal plane. TMJ: convex
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If muscle completes successfully, it will create a file seqs.afa containing the alignment. By default, output is created in "aligned FASTA" format (hence the .afa extension). This is just like regular FASTA except that gaps are added in order to align the sequences. This is a nice format for computers but not very readable for
The actual sequence can be more precisely determined by other approaches including manual DNA sequencing methods well known in the art. The polynucleotide construct of the invention can be delivered to the interstitial space of tissues within the an animal, including of muscle, skin, brain, lung, liver, spleen, bone
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