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The following points are guidelines in designing de novo or native-based peptides that have a composition that favors synthesis, purification, storage and solubility. Cysteine and methionine are susceptible to rapid oxidation, which can negatively influence the cleavage of protecting groups during synthesis and the
Peptide solubility: basic guidelines on solubilizing your Sigma peptide.
For best results, test peptide solubility with a small amount of product. Peptides shorter than five residues are usually soluble in water or aqueous buffer, except when the entire sequence consists of hydrophobic amino acids (e.g., W, L, I, F, M, V, Y).
For all peptides, usually water or diluted PBS solubilizes the peptide, however after final lyophilization and dependent upon your particular requirements, you may find it necessary to take other actions to solubilize your peptide. Normally, many peptides are easily solubilized as long as you keep the primary amino acid
Peptides are shipped at room temperature, and are highly stable at lyophilized form in sealed bags (Why in sealed bags?). Peptides should not be kept in solution for long periods of time. Peptide storage guidelines: For long-term storage, peptides should be stored in lyophilized form at -20°C, or preferably at -80°C with
1 Jan 2017 Therefore these criteria are simply meant to be guidelines for peptide selection, and some relaxation of the inclusion criteria may be necessary to .. for concentration determination; however, for peptides with well-characterized solubility, UV absorbance using pre-defined extinction coefficients may be a
A set of simple rules was derived based on these observations allowing prediction of protein solubility with 65% accuracy. A year later Bertone et Considering all proteins with known 3D structure soluble by definition, another dataset of soluble proteins (PDB-Soluble) was built from the PDB database (release 1.XII.2005).
Peptides containing more than 50% hydrophobic residues (W, L, I, F, M, V, Y, P, A) are generally poorly soluble in aqueous solutions. We recommend to dissolve the peptide in 100% ogranic solvent (DMSO, DMF or acetonitrile). Once it is solubilized, add water slowly or buffer up to the desired concentration is reached.
15 Nov 2014 While solution formulations are widely preferred, suspension formulations may be necessary when peptide solubility is insufficient to achieve a fully soluble . However, this example illustrates how a thorough evaluation of peptide stability can help guide and optimize the formulation development strategy.

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