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what is an example of a gene of interest that might be engineered into a plasmid
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Advances in technology, starting with making recombinant DNA. Genetic Engineering--The direct manipulation of genes for practical purposes. As the bacteria reproduce themselves by fission, the plasmid and the selected gene are also being cloned.
2) to make many copies of the gene itself for study. Restriction enzymes. Used to cut DNA at specific sequence of nucleotides. They are used in the creation of recombinant DNA. Recognition Site. a specific sequence within double-stranded DNA that a restriction endonuclease recognizes and cuts. Found in opposite
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allows a cloned eukaryotic gene to function in a bacterial host; a cloning vector that contains a highly active bacterial promoter upstream of a restriction site where the eukaryotic gene can be inserted into the correct reading frame. yeast artificial chromosome (YAC). essentials of a eukaryotic chromosome (DNA origin of
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