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User Manual. pYES2/CT, pYES3/CT, and. pYC2/CT. Yeast expression vectors with. C-terminal tags and auxotrophic selection markers. Catalog no. V8251-20, V8253-20, and V8255-20. Rev. date: 30 November 2009. Manual part no. 25-0304. MAN0000128
Invitrogen pYES2/CT Yeast Expression Vector 20µg Life Sciences:Molecular Biology Reagents and Kits:DNA Vectors. Features the URA3 gene for selection in yeast; pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification missing translation for 'Manuals & Protocols'.
7 Apr 2004 pYES2.1 TOPO. ®. TA Expression Kit. Five-minute cloning of Taq polymerase-amplified PCR products for regulated expression in Saccharomyces cerevisiae. Catalog no. K4150-01 DNA for automated or manual sequencing, we recommend the S.N.A.P.™ MiniPrep. Kit (Catalog no. K1900-01) or the
USER GUIDE. For Research Use Only. Not for human or animal therapeutic or diagnostic use. pYES2/NT A, B, and C. pYC2/NT A, B, and C. Yeast expression vectors with N-terminal tags. Catalog numbers V8252-20 and V8256-20. Revision date 9 July 2012. Publication Part number 25-0303. MAN0000127
5 Dec 2008 Invitrogen offers a variety of products that are suitable for use with the pYes2 plasmid. Ordering information is provided below. For detailed instructions on how to use any of the accessory products, refer to the manual provided with each product. For more information, refer to www.invitrogen.com or contact
19 ноя 2015 pYES2 Cat. no. V825–20 Version K 5 December 2008 28-0053 User Manual ii Table of Contents Table of Contents . iii Important Information . iv
12 Mar 2007 A review of the Protein Expression In Yeast Using INVSc1 And pYES2/CT From Invitrogen . All plasmids vary in size between 4.6 and 6 kb except for pYES2-DEST52, which is 7.8 kb. The manual clearly describes each step and if you stick to the protocol, there is a good chance you will be successful.
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast The pYES2 vector kit includes 20 µg of expression vector, a TOP10F? E. coli stab, and an INVSc1 yeast stab. Store the vector at -20°C. Store the TOP10F? Manuals & protocols
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