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LSR II Quick Reference & Troubleshooting Guide. Startup. Ensure that you signed up for instrument use using the on-line calendar (even if it is night or weekend). Before switching on (or when starting your run):. Check sheath reservoir. A full tank provides about 3 hours' run time. Refill as necessary from large plastic
Only required if you are using the LSR II on the weekend or on public holidays. 1. Turn on computer and log in. 2. Start BD FACSDiva software by double?clicking the shortcut on the desktop, and log in to the software. Use your personal username and password to log in to the FACSDiva software. 3. Turn the flow cytometer
It is also advised to run a series of 3 cleans of 5 minutes each before starting after priming the machine (ringed). The tubes are in a rack at the side of the LSR II and run them in the order of. Bleach, FACS Rinse and Deionised Water. Top up the tubes from the wash bottles if required. 11. Place the tube on the sample
LSR II Users Guide. Stanford Shared FACS Facility. 06/10/13. A configuration sheet is located on the. Website in the Instrument Overview area. Do I need to run beads User can purchase compensation beads from BD or Invitrogen 7 ) Follow the onscreen instructions and check the instruments laser delays, area scaling.
2002 Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise,
3 Feb 2003 Chapter 4 and Chapter 5 provide application-specific instructions for DNA analysis and calcium flux. • Chapter 6 describes maintenance procedures. • Chapter 7 details instrument troubleshooting. You can find a technical overview of BD LSR II flow cytometer fluidics, optics in Appendix A, a sample quality
This guide contains instructions for using BD FACSDiva™ software version 6.0 and later with BD™ LSR II flow cytometers. Workflow Overview. The following figure shows the steps for daily workflow using BD FACSDiva software. Before starting your daily workflow, ensure that your lab's software administrator has performed
FACS Facility LSRII User Guide. Location: 701 Acquisition on LSRII Cytometer – Approximately 2 x 10^7 cells per mL (range 0.5-?. 2 x 10^7) BD LSR II. This instrument is a multi-?parameter cell analyzer. Users operate it after basic training in operation, maintenance, and troubleshooting. It runs BD FACS DiVa software.
The BD LSR II flow cytometer is fully configurable with one to four fixed-aligned, air-cooled lasers. By distributing a large number of fluorochromes over four lasers, you reduce spectral overlap and improve sensitivity. Fixed alignment provides consistent and stable day-to-day performance. The three new solid state lasers
Canada. Toll Free (888) 259-0187. Fax (888) 229-9918. Tel (65) 6-861-0633. Tel (905) 542-8028 canada@bd.com bdbiosciences.com. Part No. 642221 Rev A. June 2007 help.biosciences@europe.bd.com. Tel 81-24-593-5405. Fax 81-24-593-5761. Fax (52) 55 5999 8288 facservice@bd.com. BD LSR II. User's Guide
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