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$Serum collection protocol mice: >> http://bit.ly/2eGYMrN << (download) serum separation from blood protocol mouse blood collection tubes serum separation from blood centrifugation mouse blood plasma collection protocol separation of plasma from blood by centrifugation how to separate serum from blood without a centrifuge separating plasma from whole blood centrifuge serum collection procedure 7 Nov 2013 Does anyone have a good protocol for how to collect and process the . Unless plasma is needed, serum collected in regular vials is the Does anyone have experience wthdrawing blood of mice (and keep to coagulate? and what speed do you centrifuge to collect the serum? 17 May 2012 This is my protocol for sampling serum from mice (or rat): 1. Take a blood from vena cava with syringe. 2. Put the blood in 1.5 mL E-tube, Snip end of mouse tail (~ 0.5 cm) and collect blood in a capillary tube as For serum, allow the blood to clot for at least 30 minutes before centrifugation for 5 Please refer to the particular protocol published by each commercial supplier for approved protocol in which the animals are used. Some IACUCs require justifi- cation for collecting blood from the orbital sinus in mice that are intended to. Keywords: Consensus, Serum collection, Plasma collection, Standard sets collected under varying protocols in combination with prospectively collected 9 Jul 2014 Blood collection and plasma and serum preparation from mouse retro-orbital plexuses.v1. Procedure. 1. Prepare all of sterile surgical Blood, plasma and serum collection. Blood is collected at 6-7.5, 12-13.5 and 18-19.5 months. Blood collection will follow four different protocols based on the different experimental objectives. Mice are chipped after being used for Blood-1, 2 protocols. Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the resulting supernatant, designated serum, is carefully removed using a Pasteur pipette.$